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Viva C8 300Å Series
Restek Viva LC Columns C8 Header Picture

Restek Viva™ Wide Pore 300 Å C8 HPLC Columns

Optimized C8 Selectivity for Protein and Peptide Analysis

The Restek Viva™ Wide Pore 300 Å C8 HPLC column series has been developed specifically for the reversed-phase separation of proteins, peptides, and other high molecular weight biomolecules. Combining a high-purity silica support with an optimized 300 Å pore structure and a conventional monomeric C8 stationary phase, these columns deliver high efficiency, excellent reproducibility, and reliable chromatographic performance for demanding bioanalytical applications.

Designed for both HPLC and LC-MS workflows, Viva C8 columns provide an ideal balance between retention and elution strength, making them an excellent choice for peptide mapping, protein characterization, and routine biopharmaceutical analysis.

Technical Specifications

Property Specification
Stationary Phase Monomeric C8 (USP L7)
Pore Size 300 Å
Particle Sizes 3 µm and 5 µm
Surface Area 100 m²/g
Carbon Load 5%
End-Capped Yes
pH Range 2.5–8.0

Maximum Operating Temperature 80 °C

Find more information in the Restek Viva LC Columns Brochure.

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36 Products
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Product Number Description Price Specifications
RT-951350210 Viva C8 300Å 5µm, 4.0 x 10mm Guard Cartridge (Trident), pk.3
EUR 306.00

Will be procured as soon as possible

Restek Viva C8 Guard Cartridges
  • Product Number: RT-951350210
  • Manufacturer: Restek
  • Brand: Viva
  • Selectivity: C8
  • Particle Size: 5.0 µm
  • Pore Volume: 0.82 mL/g
  • Pore Size: 300 Å
  • Carbon Load: 5.0 %
  • Endcapping: yes
  • Column Length: 10 mm
  • Column ID: 4.0 mm
  • Hardware Material: Stainless Steel
  • Hardware Type: Guard Cartridge
  • Application: Peptides & Proteins
  • USP Code: L7
  • Temperature Range: 80 °C
  • pH Range: 2.5-8
  • Particle: Silica
  • Particle Shape: Spherical
  • Particle Type: Fully Porous
  • Surface Area: 100 m2/g
  • Bonding Type: monomeric
  • Accessories: Guard Cartridge
  • Package Size: 3

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Price:
EUR 306.00

Will be procured as soon as possible

RT-951350212 Viva C8 300Å 5µm, 2.1 x 10mm Guard Cartridge (Trident), pk.3
EUR 254.00

Will be procured as soon as possible

Restek Viva C8 Guard Cartridges
  • Product Number: RT-951350212
  • Manufacturer: Restek
  • Brand: Viva
  • Selectivity: C8
  • Particle Size: 5.0 µm
  • Pore Volume: 0.82 mL/g
  • Pore Size: 300 Å
  • Carbon Load: 5.0 %
  • Endcapping: yes
  • Column Length: 10 mm
  • Column ID: 2.1 mm
  • Hardware Material: Stainless Steel
  • Hardware Type: Guard Cartridge
  • Application: Peptides & Proteins
  • USP Code: L7
  • Temperature Range: 80 °C
  • pH Range: 2.5-8
  • Particle: Silica
  • Particle Shape: Spherical
  • Particle Type: Fully Porous
  • Surface Area: 100 m2/g
  • Bonding Type: monomeric
  • Accessories: Guard Cartridge
  • Package Size: 3

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Price:
EUR 254.00

Will be procured as soon as possible

RT-9513511 Viva C8 300Å 5µm, 1.0 x 100mm, ea.
EUR 651.00

Will be procured as soon as possible

Restek Viva C8 LC Columns
  • Product Number: RT-9513511
  • Manufacturer: Restek
  • Brand: Viva
  • Selectivity: C8
  • Particle Size: 5.0 µm
  • Pore Volume: 0.82 mL/g
  • Pore Size: 300 Å
  • Carbon Load: 5.0 %
  • Endcapping: yes
  • Column Length: 100 mm
  • Column ID: 1.0 mm
  • Hardware Material: Stainless Steel
  • Hardware Type: Analytical Column
  • Application: Peptides & Proteins
  • USP Code: L7
  • Temperature Range: 80 °C
  • pH Range: 2.5-8
  • Particle: Silica
  • Particle Shape: Spherical
  • Particle Type: Fully Porous
  • Surface Area: 100 m2/g
  • Bonding Type: monomeric
  • Package Size: 1

Questions about the article Questions about the article To the product details To the product details

Price:
EUR 651.00

Will be procured as soon as possible

RT-9513512 Viva C8 300Å 5µm, 2.1 x 100mm, ea.
EUR 632.00

Will be procured as soon as possible

Restek Viva C8 LC Columns
  • Product Number: RT-9513512
  • Manufacturer: Restek
  • Brand: Viva
  • Selectivity: C8
  • Particle Size: 5.0 µm
  • Pore Volume: 0.82 mL/g
  • Pore Size: 300 Å
  • Carbon Load: 5.0 %
  • Endcapping: yes
  • Column Length: 100 mm
  • Column ID: 2.1 mm
  • Hardware Material: Stainless Steel
  • Hardware Type: Analytical Column
  • Application: Peptides & Proteins
  • USP Code: L7
  • Temperature Range: 80 °C
  • pH Range: 2.5-8
  • Particle: Silica
  • Particle Shape: Spherical
  • Particle Type: Fully Porous
  • Surface Area: 100 m2/g
  • Bonding Type: monomeric
  • Package Size: 1

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Price:
EUR 632.00

Will be procured as soon as possible

RT-9513512-700 Viva C8 300Å 5µm, 2.1 x 100mm (Trident), ea. Price on request
Restek Viva C8 LC Columns
  • Product Number: RT-9513512-700
  • Manufacturer: Restek
  • Brand: Viva
  • Selectivity: C8
  • Particle Size: 5.0 µm
  • Pore Volume: 0.82 mL/g
  • Pore Size: 300 Å
  • Carbon Load: 5.0 %
  • Endcapping: yes
  • Column Length: 100 mm
  • Column ID: 2.1 mm
  • Hardware Material: Stainless Steel
  • Hardware Type: Analytical Column
  • Application: Peptides & Proteins
  • USP Code: L7
  • Temperature Range: 80 °C
  • pH Range: 2.5-8
  • Particle: Silica
  • Particle Shape: Spherical
  • Particle Type: Fully Porous
  • Surface Area: 100 m2/g
  • Bonding Type: monomeric
  • Package Size: 1

Questions about the article Questions about the article To the product details To the product details

Price on request
RT-9513515 Viva C8 300Å 5µm, 4.6 x 100mm, ea.
EUR 895.00

Will be procured as soon as possible

Restek Viva C8 LC Columns
  • Product Number: RT-9513515
  • Manufacturer: Restek
  • Brand: Viva
  • Selectivity: C8
  • Particle Size: 5.0 µm
  • Pore Volume: 0.82 mL/g
  • Pore Size: 300 Å
  • Carbon Load: 5.0 %
  • Endcapping: yes
  • Column Length: 100 mm
  • Column ID: 4.6 mm
  • Hardware Material: Stainless Steel
  • Hardware Type: Analytical Column
  • Application: Peptides & Proteins
  • USP Code: L7
  • Temperature Range: 80 °C
  • pH Range: 2.5-8
  • Particle: Silica
  • Particle Shape: Spherical
  • Particle Type: Fully Porous
  • Surface Area: 100 m2/g
  • Bonding Type: monomeric
  • Package Size: 1

Questions about the article Questions about the article To the product details To the product details

Price:
EUR 895.00

Will be procured as soon as possible

RT-9513515-700 Viva C8 300Å 5µm, 4.6 x 100mm (Trident), ea. Price on request
Restek Viva C8 LC Columns
  • Product Number: RT-9513515-700
  • Manufacturer: Restek
  • Brand: Viva
  • Selectivity: C8
  • Particle Size: 5.0 µm
  • Pore Volume: 0.82 mL/g
  • Pore Size: 300 Å
  • Carbon Load: 5.0 %
  • Endcapping: yes
  • Column Length: 100 mm
  • Column ID: 4.6 mm
  • Hardware Material: Stainless Steel
  • Hardware Type: Analytical Column
  • Application: Peptides & Proteins
  • USP Code: L7
  • Temperature Range: 80 °C
  • pH Range: 2.5-8
  • Particle: Silica
  • Particle Shape: Spherical
  • Particle Type: Fully Porous
  • Surface Area: 100 m2/g
  • Bonding Type: monomeric
  • Package Size: 1

Questions about the article Questions about the article To the product details To the product details

Price on request
RT-951351E Viva C8 300Å 5µm, 3.0 x 100mm, ea.
EUR 925.00

Will be procured as soon as possible

Restek Viva C8 LC Columns
  • Product Number: RT-951351E
  • Manufacturer: Restek
  • Brand: Viva
  • Selectivity: C8
  • Particle Size: 5.0 µm
  • Pore Volume: 0.82 mL/g
  • Pore Size: 300 Å
  • Carbon Load: 5.0 %
  • Endcapping: yes
  • Column Length: 100 mm
  • Column ID: 3.0 mm
  • Hardware Material: Stainless Steel
  • Hardware Type: Analytical Column
  • Application: Peptides & Proteins
  • USP Code: L7
  • Temperature Range: 80 °C
  • pH Range: 2.5-8
  • Particle: Silica
  • Particle Shape: Spherical
  • Particle Type: Fully Porous
  • Surface Area: 100 m2/g
  • Bonding Type: monomeric
  • Package Size: 1

Questions about the article Questions about the article To the product details To the product details

Price:
EUR 925.00

Will be procured as soon as possible

RT-9513522 Viva C8 300Å 5µm, 2.1 x 200mm, ea.
EUR 962.00

Will be procured as soon as possible

Restek Viva C8 LC Columns
  • Product Number: RT-9513522
  • Manufacturer: Restek
  • Brand: Viva
  • Selectivity: C8
  • Particle Size: 5.0 µm
  • Pore Volume: 0.82 mL/g
  • Pore Size: 300 Å
  • Carbon Load: 5.0 %
  • Endcapping: yes
  • Column Length: 200 mm
  • Column ID: 2.1 mm
  • Hardware Material: Stainless Steel
  • Hardware Type: Analytical Column
  • Application: Peptides & Proteins
  • USP Code: L7
  • Temperature Range: 80 °C
  • pH Range: 2.5-8
  • Particle: Silica
  • Particle Shape: Spherical
  • Particle Type: Fully Porous
  • Surface Area: 100 m2/g
  • Bonding Type: monomeric
  • Package Size: 1

Questions about the article Questions about the article To the product details To the product details

Price:
EUR 962.00

Will be procured as soon as possible

RT-9513522-700 Viva C8 300Å 5µm, 2.1 x 200mm (Trident), ea. Price on request
Restek Viva C8 LC Columns
  • Product Number: RT-9513522-700
  • Manufacturer: Restek
  • Brand: Viva
  • Selectivity: C8
  • Particle Size: 5.0 µm
  • Pore Volume: 0.82 mL/g
  • Pore Size: 300 Å
  • Carbon Load: 5.0 %
  • Endcapping: yes
  • Column Length: 200 mm
  • Column ID: 2.1 mm
  • Hardware Material: Stainless Steel
  • Hardware Type: Analytical Column
  • Application: Peptides & Proteins
  • USP Code: L7
  • Temperature Range: 80 °C
  • pH Range: 2.5-8
  • Particle: Silica
  • Particle Shape: Spherical
  • Particle Type: Fully Porous
  • Surface Area: 100 m2/g
  • Bonding Type: monomeric
  • Package Size: 1

Questions about the article Questions about the article To the product details To the product details

Price on request
RT-9513525 Viva C8 300Å 5µm, 4.6 x 200mm, ea.
EUR 1,401.00

Will be procured as soon as possible

Restek Viva C8 LC Columns
  • Product Number: RT-9513525
  • Manufacturer: Restek
  • Brand: Viva
  • Selectivity: C8
  • Particle Size: 5.0 µm
  • Pore Volume: 0.82 mL/g
  • Pore Size: 300 Å
  • Carbon Load: 5.0 %
  • Endcapping: yes
  • Column Length: 200 mm
  • Column ID: 4.6 mm
  • Hardware Material: Stainless Steel
  • Hardware Type: Analytical Column
  • Application: Peptides & Proteins
  • USP Code: L7
  • Temperature Range: 80 °C
  • pH Range: 2.5-8
  • Particle: Silica
  • Particle Shape: Spherical
  • Particle Type: Fully Porous
  • Surface Area: 100 m2/g
  • Bonding Type: monomeric
  • Package Size: 1

Questions about the article Questions about the article To the product details To the product details

Price:
EUR 1,401.00

Will be procured as soon as possible

RT-9513525-700 Viva C8 300Å 5µm, 4.6 x 200mm (Trident), ea. Price on request
Restek Viva C8 LC Columns
  • Product Number: RT-9513525-700
  • Manufacturer: Restek
  • Brand: Viva
  • Selectivity: C8
  • Particle Size: 5.0 µm
  • Pore Volume: 0.82 mL/g
  • Pore Size: 300 Å
  • Carbon Load: 5.0 %
  • Endcapping: yes
  • Column Length: 200 mm
  • Column ID: 4.6 mm
  • Hardware Material: Stainless Steel
  • Hardware Type: Analytical Column
  • Application: Peptides & Proteins
  • USP Code: L7
  • Temperature Range: 80 °C
  • pH Range: 2.5-8
  • Particle: Silica
  • Particle Shape: Spherical
  • Particle Type: Fully Porous
  • Surface Area: 100 m2/g
  • Bonding Type: monomeric
  • Package Size: 1

Questions about the article Questions about the article To the product details To the product details

Price on request
RT-951352E Viva C8 300Å 5µm, 3.0 x 200mm, ea.
EUR 1,541.00

Will be procured as soon as possible

Restek Viva C8 LC Columns
  • Product Number: RT-951352E
  • Manufacturer: Restek
  • Brand: Viva
  • Selectivity: C8
  • Particle Size: 5.0 µm
  • Pore Volume: 0.82 mL/g
  • Pore Size: 300 Å
  • Carbon Load: 5.0 %
  • Endcapping: yes
  • Column Length: 200 mm
  • Column ID: 3.0 mm
  • Hardware Material: Stainless Steel
  • Hardware Type: Analytical Column
  • Application: Peptides & Proteins
  • USP Code: L7
  • Temperature Range: 80 °C
  • pH Range: 2.5-8
  • Particle: Silica
  • Particle Shape: Spherical
  • Particle Type: Fully Porous
  • Surface Area: 100 m2/g
  • Bonding Type: monomeric
  • Package Size: 1

Questions about the article Questions about the article To the product details To the product details

Price:
EUR 1,541.00

Will be procured as soon as possible

RT-9513532 Viva C8 300Å 5µm, 2.1 x 30mm, ea.
EUR 569.00

Will be procured as soon as possible

Restek Viva C8 LC Columns
  • Product Number: RT-9513532
  • Manufacturer: Restek
  • Brand: Viva
  • Selectivity: C8
  • Particle Size: 5.0 µm
  • Pore Volume: 0.82 mL/g
  • Pore Size: 300 Å
  • Carbon Load: 5.0 %
  • Endcapping: yes
  • Column Length: 30 mm
  • Column ID: 2.1 mm
  • Hardware Material: Stainless Steel
  • Hardware Type: Analytical Column
  • Application: Peptides & Proteins
  • USP Code: L7
  • Temperature Range: 80 °C
  • pH Range: 2.5-8
  • Particle: Silica
  • Particle Shape: Spherical
  • Particle Type: Fully Porous
  • Surface Area: 100 m2/g
  • Bonding Type: monomeric
  • Package Size: 1

Questions about the article Questions about the article To the product details To the product details

Price:
EUR 569.00

Will be procured as soon as possible

RT-9513532-700 Viva C8 300Å 5µm, 2.1 x 30mm (Trident), ea. Price on request
Restek Viva C8 LC Columns
  • Product Number: RT-9513532-700
  • Manufacturer: Restek
  • Brand: Viva
  • Selectivity: C8
  • Particle Size: 5.0 µm
  • Pore Volume: 0.82 mL/g
  • Pore Size: 300 Å
  • Carbon Load: 5.0 %
  • Endcapping: yes
  • Column Length: 30 mm
  • Column ID: 2.1 mm
  • Hardware Material: Stainless Steel
  • Hardware Type: Analytical Column
  • Application: Peptides & Proteins
  • USP Code: L7
  • Temperature Range: 80 °C
  • pH Range: 2.5-8
  • Particle: Silica
  • Particle Shape: Spherical
  • Particle Type: Fully Porous
  • Surface Area: 100 m2/g
  • Bonding Type: monomeric
  • Package Size: 1

Questions about the article Questions about the article To the product details To the product details

Price on request
RT-9513535 Viva C8 300Å 5µm, 4.6 x 30mm, ea.
EUR 630.00

Will be procured as soon as possible

Restek Viva C8 LC Columns
  • Product Number: RT-9513535
  • Manufacturer: Restek
  • Brand: Viva
  • Selectivity: C8
  • Particle Size: 5.0 µm
  • Pore Volume: 0.82 mL/g
  • Pore Size: 300 Å
  • Carbon Load: 5.0 %
  • Endcapping: yes
  • Column Length: 30 mm
  • Column ID: 4.6 mm
  • Hardware Material: Stainless Steel
  • Hardware Type: Analytical Column
  • Application: Peptides & Proteins
  • USP Code: L7
  • Temperature Range: 80 °C
  • pH Range: 2.5-8
  • Particle: Silica
  • Particle Shape: Spherical
  • Particle Type: Fully Porous
  • Surface Area: 100 m2/g
  • Bonding Type: monomeric
  • Package Size: 1

Questions about the article Questions about the article To the product details To the product details

Price:
EUR 630.00

Will be procured as soon as possible

RT-9513535-700 Viva C8 300Å 5µm, 4.6 x 30mm (Trident), ea. Price on request
Restek Viva C8 LC Columns
  • Product Number: RT-9513535-700
  • Manufacturer: Restek
  • Brand: Viva
  • Selectivity: C8
  • Particle Size: 5.0 µm
  • Pore Volume: 0.82 mL/g
  • Pore Size: 300 Å
  • Carbon Load: 5.0 %
  • Endcapping: yes
  • Column Length: 30 mm
  • Column ID: 4.6 mm
  • Hardware Material: Stainless Steel
  • Hardware Type: Analytical Column
  • Application: Peptides & Proteins
  • USP Code: L7
  • Temperature Range: 80 °C
  • pH Range: 2.5-8
  • Particle: Silica
  • Particle Shape: Spherical
  • Particle Type: Fully Porous
  • Surface Area: 100 m2/g
  • Bonding Type: monomeric
  • Package Size: 1

Questions about the article Questions about the article To the product details To the product details

Price on request
RT-951353E Viva C8 300Å 5µm, 3.0 x 30mm, ea.
EUR 569.00

Will be procured as soon as possible

Restek Viva C8 LC Columns
  • Product Number: RT-951353E
  • Manufacturer: Restek
  • Brand: Viva
  • Selectivity: C8
  • Particle Size: 5.0 µm
  • Pore Volume: 0.82 mL/g
  • Pore Size: 300 Å
  • Carbon Load: 5.0 %
  • Endcapping: yes
  • Column Length: 30 mm
  • Column ID: 3.0 mm
  • Hardware Material: Stainless Steel
  • Hardware Type: Analytical Column
  • Application: Peptides & Proteins
  • USP Code: L7
  • Temperature Range: 80 °C
  • pH Range: 2.5-8
  • Particle: Silica
  • Particle Shape: Spherical
  • Particle Type: Fully Porous
  • Surface Area: 100 m2/g
  • Bonding Type: monomeric
  • Package Size: 1

Questions about the article Questions about the article To the product details To the product details

Price:
EUR 569.00

Will be procured as soon as possible

RT-9513551 Viva C8 300Å 5µm, 1.0 x 50mm, ea.
EUR 602.00

Will be procured as soon as possible

Restek Viva C8 LC Columns
  • Product Number: RT-9513551
  • Manufacturer: Restek
  • Brand: Viva
  • Selectivity: C8
  • Particle Size: 5.0 µm
  • Pore Volume: 0.82 mL/g
  • Pore Size: 300 Å
  • Carbon Load: 5.0 %
  • Endcapping: yes
  • Column Length: 50 mm
  • Column ID: 1.0 mm
  • Hardware Material: Stainless Steel
  • Hardware Type: Analytical Column
  • Application: Peptides & Proteins
  • USP Code: L7
  • Temperature Range: 80 °C
  • pH Range: 2.5-8
  • Particle: Silica
  • Particle Shape: Spherical
  • Particle Type: Fully Porous
  • Surface Area: 100 m2/g
  • Bonding Type: monomeric
  • Package Size: 1

Questions about the article Questions about the article To the product details To the product details

Price:
EUR 602.00

Will be procured as soon as possible

RT-9513552 Viva C8 300Å 5µm, 2.1 x 50mm, ea.
EUR 569.00

Will be procured as soon as possible

Restek Viva C8 LC Columns
  • Product Number: RT-9513552
  • Manufacturer: Restek
  • Brand: Viva
  • Selectivity: C8
  • Particle Size: 5.0 µm
  • Pore Volume: 0.82 mL/g
  • Pore Size: 300 Å
  • Carbon Load: 5.0 %
  • Endcapping: yes
  • Column Length: 50 mm
  • Column ID: 2.1 mm
  • Hardware Material: Stainless Steel
  • Hardware Type: Analytical Column
  • Application: Peptides & Proteins
  • USP Code: L7
  • Temperature Range: 80 °C
  • pH Range: 2.5-8
  • Particle: Silica
  • Particle Shape: Spherical
  • Particle Type: Fully Porous
  • Surface Area: 100 m2/g
  • Bonding Type: monomeric
  • Package Size: 1

Questions about the article Questions about the article To the product details To the product details

Price:
EUR 569.00

Will be procured as soon as possible

RT-9513552-700 Viva C8 300Å 5µm, 2.1 x 50mm (Trident), ea. Price on request
Restek Viva C8 LC Columns
  • Product Number: RT-9513552-700
  • Manufacturer: Restek
  • Brand: Viva
  • Selectivity: C8
  • Particle Size: 5.0 µm
  • Pore Volume: 0.82 mL/g
  • Pore Size: 300 Å
  • Carbon Load: 5.0 %
  • Endcapping: yes
  • Column Length: 50 mm
  • Column ID: 2.1 mm
  • Hardware Material: Stainless Steel
  • Hardware Type: Analytical Column
  • Application: Peptides & Proteins
  • USP Code: L7
  • Temperature Range: 80 °C
  • pH Range: 2.5-8
  • Particle: Silica
  • Particle Shape: Spherical
  • Particle Type: Fully Porous
  • Surface Area: 100 m2/g
  • Bonding Type: monomeric
  • Package Size: 1

Questions about the article Questions about the article To the product details To the product details

Price on request
RT-9513555 Viva C8 300Å 5µm, 4.6 x 50mm, ea.
EUR 822.00

Will be procured as soon as possible

Restek Viva C8 LC Columns
  • Product Number: RT-9513555
  • Manufacturer: Restek
  • Brand: Viva
  • Selectivity: C8
  • Particle Size: 5.0 µm
  • Pore Volume: 0.82 mL/g
  • Pore Size: 300 Å
  • Carbon Load: 5.0 %
  • Endcapping: yes
  • Column Length: 50 mm
  • Column ID: 4.6 mm
  • Hardware Material: Stainless Steel
  • Hardware Type: Analytical Column
  • Application: Peptides & Proteins
  • USP Code: L7
  • Temperature Range: 80 °C
  • pH Range: 2.5-8
  • Particle: Silica
  • Particle Shape: Spherical
  • Particle Type: Fully Porous
  • Surface Area: 100 m2/g
  • Bonding Type: monomeric
  • Package Size: 1

Questions about the article Questions about the article To the product details To the product details

Price:
EUR 822.00

Will be procured as soon as possible

RT-9513555-700 Viva C8 300Å 5µm, 4.6 x 50mm (Trident), ea. Price on request
Restek Viva C8 LC Columns
  • Product Number: RT-9513555-700
  • Manufacturer: Restek
  • Brand: Viva
  • Selectivity: C8
  • Particle Size: 5.0 µm
  • Pore Volume: 0.82 mL/g
  • Pore Size: 300 Å
  • Carbon Load: 5.0 %
  • Endcapping: yes
  • Column Length: 50 mm
  • Column ID: 4.6 mm
  • Hardware Material: Stainless Steel
  • Hardware Type: Analytical Column
  • Application: Peptides & Proteins
  • USP Code: L7
  • Temperature Range: 80 °C
  • pH Range: 2.5-8
  • Particle: Silica
  • Particle Shape: Spherical
  • Particle Type: Fully Porous
  • Surface Area: 100 m2/g
  • Bonding Type: monomeric
  • Package Size: 1

Questions about the article Questions about the article To the product details To the product details

Price on request
RT-951355E Viva C8 300Å 5µm, 3.0 x 50mm, ea.
EUR 602.00

Will be procured as soon as possible

Restek Viva C8 LC Columns
  • Product Number: RT-951355E
  • Manufacturer: Restek
  • Brand: Viva
  • Selectivity: C8
  • Particle Size: 5.0 µm
  • Pore Volume: 0.82 mL/g
  • Pore Size: 300 Å
  • Carbon Load: 5.0 %
  • Endcapping: yes
  • Column Length: 50 mm
  • Column ID: 3.0 mm
  • Hardware Material: Stainless Steel
  • Hardware Type: Analytical Column
  • Application: Peptides & Proteins
  • USP Code: L7
  • Temperature Range: 80 °C
  • pH Range: 2.5-8
  • Particle: Silica
  • Particle Shape: Spherical
  • Particle Type: Fully Porous
  • Surface Area: 100 m2/g
  • Bonding Type: monomeric
  • Package Size: 1

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Price:
EUR 602.00

Will be procured as soon as possible

RT-9513561 Viva C8 300Å 5µm, 1.0 x 150mm, ea.
EUR 701.00

Will be procured as soon as possible

Restek Viva C8 LC Columns
  • Product Number: RT-9513561
  • Manufacturer: Restek
  • Brand: Viva
  • Selectivity: C8
  • Particle Size: 5.0 µm
  • Pore Volume: 0.82 mL/g
  • Pore Size: 300 Å
  • Carbon Load: 5.0 %
  • Endcapping: yes
  • Column Length: 150 mm
  • Column ID: 1.0 mm
  • Hardware Material: Stainless Steel
  • Hardware Type: Analytical Column
  • Application: Peptides & Proteins
  • USP Code: L7
  • Temperature Range: 80 °C
  • pH Range: 2.5-8
  • Particle: Silica
  • Particle Shape: Spherical
  • Particle Type: Fully Porous
  • Surface Area: 100 m2/g
  • Bonding Type: monomeric
  • Package Size: 1

Questions about the article Questions about the article To the product details To the product details

Price:
EUR 701.00

Will be procured as soon as possible

Optimized Wide Pore Technology

Efficient separation of large biomolecules requires chromatographic media with pores that are fully accessible to proteins and peptides. Although many commercially available materials are specified with an average pore size of 300 Å, they often contain a broad pore size distribution that limits the accessible surface area for larger analytes.

Restek manufactures Viva™ Wide Pore silica with a narrow pore size distribution centered around 300 Å. This optimized architecture provides a greater proportion of accessible surface area, allowing proteins and peptides to interact more effectively with the stationary phase. The result is improved retention, enhanced mass transfer, higher chromatographic efficiency, and excellent peak shape for large biomolecules.

Balanced Hydrophobic Retention

The Viva™ C8 stationary phase is a monomeric octylsilane (USP L7) chemistry that offers lower hydrophobic retention than traditional C18 columns. This moderate retention is particularly advantageous for larger proteins and hydrophobic peptides, allowing faster elution while maintaining excellent resolution.

Compared with C18 columns, the shorter C8 ligand can also provide improved peak shape for slightly basic analytes and shorter analysis times without compromising chromatographic performance.

Typical applications include:

  • Peptide mapping
  • Intact protein analysis
  • Protein purity testing
  • Recombinant protein characterization
  • Biopharmaceutical quality control
  • Enzymatic digest analysis
  • LC-MS method development
  • Routine bioanalytical testing

Key Features and Benefits

  • 300 Å wide pore silica optimized for proteins and peptides
  • Narrow pore size distribution for maximum accessible surface area
  • High-purity, spherical silica particles
  • Monomeric C8 stationary phase (USP L7)
  • End-capped surface chemistry for improved peak symmetry
  • Reduced hydrophobic retention compared with C18
  • Excellent recovery of large biomolecules
  • High chromatographic efficiency
  • Outstanding batch-to-batch reproducibility
  • Fully compatible with LC-UV and LC-MS methods

Reliable Performance for Biomolecule Separations

Restek manufactures every Viva™ column—from silica production and stationary phase bonding to final column packing—under carefully controlled conditions. This integrated manufacturing process ensures consistent selectivity, reproducible retention times, and dependable column performance throughout the product lifetime.

The Viva™ Wide Pore C8 column is particularly well suited for laboratories requiring robust analytical methods with excellent reproducibility for protein and peptide separations.

Typical Applications

Restek Viva™ Wide Pore 300 Å C8 columns are ideally suited for:

  • Peptide mapping and characterization
  • Therapeutic protein analysis
  • Monoclonal antibody research
  • Recombinant protein analysis
  • Protein degradation studies
  • Biomarker discovery
  • Proteomics research
  • Biopharmaceutical quality control
  • LC-MS analysis of proteins and peptides

The Ideal Choice for Fast and Efficient Biomolecule Analysis

The Restek Viva™ Wide Pore 300 Å C8 HPLC column combines optimized pore accessibility with balanced C8 selectivity to deliver reliable separations of proteins and peptides. Its reduced hydrophobic retention enables faster analyses and excellent peak shape while maintaining the resolution and reproducibility required for modern bioanalytical and biopharmaceutical laboratories.

Whether used for routine quality control or advanced method development, Viva™ C8 columns provide the robust performance and consistent results that analytical scientists expect from Restek chromatography products.

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