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Why Choose Restek Viva Wide Pore LC Columns?

Optimized for Large Biomolecules

Traditional HPLC columns with pore sizes between 60 and 150 Å often restrict access for larger molecules, reducing retention and chromatographic efficiency. The Viva Wide Pore phase utilizes a 300 Å pore structure specifically engineered to maximize accessibility for proteins, peptides, and other large analytes.

Maximum Surface Area for Enhanced Retention

The Viva silica material provides one of the highest available surface areas within the critical 250–350 Å pore range. This enables greater analyte interaction with the stationary phase, resulting in improved retention and higher chromatographic resolution.

Narrow Pore Size Distribution

Many commercially available wide pore materials contain a significant proportion of smaller pores that are inaccessible to large molecules. Viva columns feature a tightly controlled pore size distribution around the 300 Å average, ensuring consistent and efficient separations.

Excellent Peak Shape

The highly base-deactivated silica surface minimizes unwanted secondary interactions and delivers sharp, symmetrical peaks for proteins, peptides, and other biomolecules.

Ideal for LC-MS and Biopharmaceutical Workflows

Viva columns are widely used for:

  • Protein characterization
  • Peptide mapping
  • Proteomics research
  • Biotherapeutic development
  • PEGylation reaction monitoring
  • Biomarker analysis
  • Biopharmaceutical quality control
  • LC-MS and LC-MS/MS applications

Key Advantages

  • Exceptional retention of proteins and peptides
  • Optimized 300 Å wide pore structure
  • High accessible surface area for large molecules
  • Superior chromatographic resolution
  • Excellent peak symmetry
  • Outstanding lot-to-lot reproducibility
  • Robust silica-based stationary phase
  • Compatible with HPLC and LC-MS systems

Designed for Modern Biopharmaceutical Analysis

As therapeutic proteins, peptides, and PEGylated biomolecules continue to grow in importance, chromatographic methods require stationary phases specifically designed for large molecule separations. The Restek Viva Wide Pore LC phase addresses this challenge by providing superior pore accessibility, high retention, and excellent reproducibility for complex biomolecular analyses.

The column technology is particularly effective for monitoring PEGylation reactions, peptide purification workflows, and protein characterization studies where conventional reversed-phase columns often fail to provide sufficient retention and resolution.

Applications

Protein Analysis

Reliable separation and characterization of intact proteins and protein fragments.

Peptide Mapping

High-resolution peptide separations for proteomics and pharmaceutical research.

PEGylation Monitoring

Efficient monitoring of PEGylated peptides and therapeutic proteins during development and manufacturing.

Biopharmaceutical Quality Control

Robust and reproducible analysis of biologics, biosimilars, and protein-based therapeutics.

LC-MS Workflows

Enhanced sensitivity and chromatographic performance for biomolecule identification and quantification.

The Ideal Choice for Biomolecule Separations

The Restek Viva Wide Pore LC phase combines advanced silica technology, optimized pore architecture, and proven chromatographic performance to deliver reliable separations of large biomolecules. For laboratories focused on proteins, peptides, biologics, and biotherapeutics, Viva columns provide the retention, resolution, and reproducibility required for modern analytical workflows.

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