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Viva C4 300Å Series
Restek Viva LC Columns C4 Header Picture

Restek Viva™ Wide Pore 300 Å C4 HPLC Columns

Optimized C4 Columns for Protein and Peptide Separations

The Restek Viva™ Wide Pore 300 Å C4 HPLC column series is specifically designed for the reversed-phase analysis of proteins, peptides, and other high molecular weight biomolecules. Combining an optimized 300 Å wide pore silica with a monomeric C4 bonded phase, these columns provide the ideal balance of retention, recovery, and resolution for large biomolecules.

The moderate hydrophobicity of the C4 stationary phase makes it particularly suitable for intact proteins and highly hydrophobic biomolecules, where stronger alkyl phases may result in excessive retention. Viva C4 columns deliver excellent chromatographic performance for both routine bioanalytical testing and advanced biopharmaceutical characterization.

Technical Specifications

Property Specification
Stationary Phase Monomeric C4 (USP L26)
Pore Size 300 Å
Particle Sizes 5 µm
Surface Area 100 m²/g
Carbon Load 3.5%
End-Capped Yes
pH Range 2.5–8.0

Maximum Operating Temperature 80 °C

Find more information in the Restek Viva LC Columns Brochure.

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36 Products
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Product Number Description Price Specifications
RT-951250210 Viva C4 300Å 5µm, 4.0 x 10mm Guard Cartridge (Trident), pk.3
EUR 269.00

Will be procured as soon as possible

Restek Viva C4 Guard Cartridges
  • Product Number: RT-951250210
  • Manufacturer: Restek
  • Brand: Viva
  • Selectivity: C4
  • Particle Size: 5.0 µm
  • Pore Volume: 0.82 mL/g
  • Pore Size: 300 Å
  • Carbon Load: 3.5 %
  • Endcapping: yes
  • Column Length: 10 mm
  • Column ID: 4.0 mm
  • Hardware Material: Stainless Steel
  • Hardware Type: Guard Cartridge
  • Application: Peptides & Proteins
  • USP Code: L26
  • Temperature Range: 80 °C
  • pH Range: 2.5-8
  • Particle: Silica
  • Particle Shape: Spherical
  • Particle Type: Fully Porous
  • Surface Area: 100 m2/g
  • Bonding Type: monomeric
  • Accessories: Guard Cartridge
  • Package Size: 3

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Price:
EUR 269.00

Will be procured as soon as possible

RT-951250212 Viva C4 300Å 5µm, 2.1 x 10mm Guard Cartridge (Trident), pk.3
EUR 254.00

Will be procured as soon as possible

Restek Viva C4 Guard Cartridges
  • Product Number: RT-951250212
  • Manufacturer: Restek
  • Brand: Viva
  • Selectivity: C4
  • Particle Size: 5.0 µm
  • Pore Volume: 0.82 mL/g
  • Pore Size: 300 Å
  • Carbon Load: 3.5 %
  • Endcapping: yes
  • Column Length: 10 mm
  • Column ID: 2.1 mm
  • Hardware Material: Stainless Steel
  • Hardware Type: Guard Cartridge
  • Application: Peptides & Proteins
  • USP Code: L26
  • Temperature Range: 80 °C
  • pH Range: 2.5-8
  • Particle: Silica
  • Particle Shape: Spherical
  • Particle Type: Fully Porous
  • Surface Area: 100 m2/g
  • Bonding Type: monomeric
  • Accessories: Guard Cartridge
  • Package Size: 3

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Price:
EUR 254.00

Will be procured as soon as possible

RT-9512511 Viva C4 300Å 5µm, 1.0 x 100mm, ea.
EUR 651.00

Will be procured as soon as possible

Restek Viva C4 LC Columns
  • Product Number: RT-9512511
  • Manufacturer: Restek
  • Brand: Viva
  • Selectivity: C4
  • Particle Size: 5.0 µm
  • Pore Volume: 0.82 mL/g
  • Pore Size: 300 Å
  • Carbon Load: 3.5 %
  • Endcapping: yes
  • Column Length: 100 mm
  • Column ID: 1.0 mm
  • Hardware Material: Stainless Steel
  • Hardware Type: Analytical Column
  • Application: Peptides & Proteins
  • USP Code: L26
  • Temperature Range: 80 °C
  • pH Range: 2.5-8
  • Particle: Silica
  • Particle Shape: Spherical
  • Particle Type: Fully Porous
  • Surface Area: 100 m2/g
  • Bonding Type: monomeric
  • Package Size: 1

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Price:
EUR 651.00

Will be procured as soon as possible

RT-9512512 Viva C4 300Å 5µm, 2.1 x 100mm, ea.
EUR 620.00

Will be procured as soon as possible

Restek Viva C4 LC Columns
  • Product Number: RT-9512512
  • Manufacturer: Restek
  • Brand: Viva
  • Selectivity: C4
  • Particle Size: 5.0 µm
  • Pore Volume: 0.82 mL/g
  • Pore Size: 300 Å
  • Carbon Load: 3.5 %
  • Endcapping: yes
  • Column Length: 100 mm
  • Column ID: 2.1 mm
  • Hardware Material: Stainless Steel
  • Hardware Type: Analytical Column
  • Application: Peptides & Proteins
  • USP Code: L26
  • Temperature Range: 80 °C
  • pH Range: 2.5-8
  • Particle: Silica
  • Particle Shape: Spherical
  • Particle Type: Fully Porous
  • Surface Area: 100 m2/g
  • Bonding Type: monomeric
  • Package Size: 1

Questions about the article Questions about the article To the product details To the product details

Price:
EUR 620.00

Will be procured as soon as possible

RT-9512512-700 Viva C4 300Å 5µm, 2.1 x 100mm (Trident), ea. Price on request
Restek Viva C4 LC Columns
  • Product Number: RT-9512512-700
  • Manufacturer: Restek
  • Brand: Viva
  • Selectivity: C4
  • Particle Size: 5.0 µm
  • Pore Volume: 0.82 mL/g
  • Pore Size: 300 Å
  • Carbon Load: 3.5 %
  • Endcapping: yes
  • Column Length: 100 mm
  • Column ID: 2.1 mm
  • Hardware Material: Stainless Steel
  • Hardware Type: Analytical Column
  • Application: Peptides & Proteins
  • USP Code: L26
  • Temperature Range: 80 °C
  • pH Range: 2.5-8
  • Particle: Silica
  • Particle Shape: Spherical
  • Particle Type: Fully Porous
  • Surface Area: 100 m2/g
  • Bonding Type: monomeric
  • Package Size: 1

Questions about the article Questions about the article To the product details To the product details

Price on request
RT-9512515 Viva C4 300Å 5µm, 4.6 x 100mm, ea.
EUR 716.00

Will be procured as soon as possible

Restek Viva C4 LC Columns
  • Product Number: RT-9512515
  • Manufacturer: Restek
  • Brand: Viva
  • Selectivity: C4
  • Particle Size: 5.0 µm
  • Pore Volume: 0.82 mL/g
  • Pore Size: 300 Å
  • Carbon Load: 3.5 %
  • Endcapping: yes
  • Column Length: 100 mm
  • Column ID: 4.6 mm
  • Hardware Material: Stainless Steel
  • Hardware Type: Analytical Column
  • Application: Peptides & Proteins
  • USP Code: L26
  • Temperature Range: 80 °C
  • pH Range: 2.5-8
  • Particle: Silica
  • Particle Shape: Spherical
  • Particle Type: Fully Porous
  • Surface Area: 100 m2/g
  • Bonding Type: monomeric
  • Package Size: 1

Questions about the article Questions about the article To the product details To the product details

Price:
EUR 716.00

Will be procured as soon as possible

RT-9512515-700 Viva C4 300Å 5µm, 4.6 x 100mm (Trident), ea. Price on request
Restek Viva C4 LC Columns
  • Product Number: RT-9512515-700
  • Manufacturer: Restek
  • Brand: Viva
  • Selectivity: C4
  • Particle Size: 5.0 µm
  • Pore Volume: 0.82 mL/g
  • Pore Size: 300 Å
  • Carbon Load: 3.5 %
  • Endcapping: yes
  • Column Length: 100 mm
  • Column ID: 4.6 mm
  • Hardware Material: Stainless Steel
  • Hardware Type: Analytical Column
  • Application: Peptides & Proteins
  • USP Code: L26
  • Temperature Range: 80 °C
  • pH Range: 2.5-8
  • Particle: Silica
  • Particle Shape: Spherical
  • Particle Type: Fully Porous
  • Surface Area: 100 m2/g
  • Bonding Type: monomeric
  • Package Size: 1

Questions about the article Questions about the article To the product details To the product details

Price on request
RT-951251E Viva C4 300Å 5µm, 3.0 x 100mm, ea.
EUR 685.00

Will be procured as soon as possible

Restek Viva C4 LC Columns
  • Product Number: RT-951251E
  • Manufacturer: Restek
  • Brand: Viva
  • Selectivity: C4
  • Particle Size: 5.0 µm
  • Pore Volume: 0.82 mL/g
  • Pore Size: 300 Å
  • Carbon Load: 3.5 %
  • Endcapping: yes
  • Column Length: 100 mm
  • Column ID: 3.0 mm
  • Hardware Material: Stainless Steel
  • Hardware Type: Analytical Column
  • Application: Peptides & Proteins
  • USP Code: L26
  • Temperature Range: 80 °C
  • pH Range: 2.5-8
  • Particle: Silica
  • Particle Shape: Spherical
  • Particle Type: Fully Porous
  • Surface Area: 100 m2/g
  • Bonding Type: monomeric
  • Package Size: 1

Questions about the article Questions about the article To the product details To the product details

Price:
EUR 685.00

Will be procured as soon as possible

RT-9512522 Viva C4 300Å 5µm, 2.1 x 200mm, ea.
EUR 727.00

Will be procured as soon as possible

Restek Viva C4 LC Columns
  • Product Number: RT-9512522
  • Manufacturer: Restek
  • Brand: Viva
  • Selectivity: C4
  • Particle Size: 5.0 µm
  • Pore Volume: 0.82 mL/g
  • Pore Size: 300 Å
  • Carbon Load: 3.5 %
  • Endcapping: yes
  • Column Length: 200 mm
  • Column ID: 2.1 mm
  • Hardware Material: Stainless Steel
  • Hardware Type: Analytical Column
  • Application: Peptides & Proteins
  • USP Code: L26
  • Temperature Range: 80 °C
  • pH Range: 2.5-8
  • Particle: Silica
  • Particle Shape: Spherical
  • Particle Type: Fully Porous
  • Surface Area: 100 m2/g
  • Bonding Type: monomeric
  • Package Size: 1

Questions about the article Questions about the article To the product details To the product details

Price:
EUR 727.00

Will be procured as soon as possible

RT-9512522-700 Viva C4 300Å 5µm, 2.1 x 200mm (Trident), ea.
EUR 760.00

Will be procured as soon as possible

Restek Viva C4 LC Columns
  • Product Number: RT-9512522-700
  • Manufacturer: Restek
  • Brand: Viva
  • Selectivity: C4
  • Particle Size: 5.0 µm
  • Pore Volume: 0.82 mL/g
  • Pore Size: 300 Å
  • Carbon Load: 3.5 %
  • Endcapping: yes
  • Column Length: 200 mm
  • Column ID: 2.1 mm
  • Hardware Material: Stainless Steel
  • Hardware Type: Analytical Column
  • Application: Peptides & Proteins
  • USP Code: L26
  • Temperature Range: 80 °C
  • pH Range: 2.5-8
  • Particle: Silica
  • Particle Shape: Spherical
  • Particle Type: Fully Porous
  • Surface Area: 100 m2/g
  • Bonding Type: monomeric
  • Package Size: 1

Questions about the article Questions about the article To the product details To the product details

Price:
EUR 760.00

Will be procured as soon as possible

RT-9512525 Viva C4 300Å 5µm, 4.6 x 200mm, ea.
EUR 1,051.00

Will be procured as soon as possible

Restek Viva C4 LC Columns
  • Product Number: RT-9512525
  • Manufacturer: Restek
  • Brand: Viva
  • Selectivity: C4
  • Particle Size: 5.0 µm
  • Pore Volume: 0.82 mL/g
  • Pore Size: 300 Å
  • Carbon Load: 3.5 %
  • Endcapping: yes
  • Column Length: 200 mm
  • Column ID: 4.6 mm
  • Hardware Material: Stainless Steel
  • Hardware Type: Analytical Column
  • Application: Peptides & Proteins
  • USP Code: L26
  • Temperature Range: 80 °C
  • pH Range: 2.5-8
  • Particle: Silica
  • Particle Shape: Spherical
  • Particle Type: Fully Porous
  • Surface Area: 100 m2/g
  • Bonding Type: monomeric
  • Package Size: 1

Questions about the article Questions about the article To the product details To the product details

Price:
EUR 1,051.00

Will be procured as soon as possible

RT-9512525-700 Viva C4 300Å 5µm, 4.6 x 200mm (Trident), ea. Price on request
Restek Viva C4 LC Columns
  • Product Number: RT-9512525-700
  • Manufacturer: Restek
  • Brand: Viva
  • Selectivity: C4
  • Particle Size: 5.0 µm
  • Pore Volume: 0.82 mL/g
  • Pore Size: 300 Å
  • Carbon Load: 3.5 %
  • Endcapping: yes
  • Column Length: 200 mm
  • Column ID: 4.6 mm
  • Hardware Material: Stainless Steel
  • Hardware Type: Analytical Column
  • Application: Peptides & Proteins
  • USP Code: L26
  • Temperature Range: 80 °C
  • pH Range: 2.5-8
  • Particle: Silica
  • Particle Shape: Spherical
  • Particle Type: Fully Porous
  • Surface Area: 100 m2/g
  • Bonding Type: monomeric
  • Package Size: 1

Questions about the article Questions about the article To the product details To the product details

Price on request
RT-951252E Viva C4 300Å 5µm, 3.0 x 200mm, ea.
EUR 1,064.00

Will be procured as soon as possible

Restek Viva C4 LC Columns
  • Product Number: RT-951252E
  • Manufacturer: Restek
  • Brand: Viva
  • Selectivity: C4
  • Particle Size: 5.0 µm
  • Pore Volume: 0.82 mL/g
  • Pore Size: 300 Å
  • Carbon Load: 3.5 %
  • Endcapping: yes
  • Column Length: 200 mm
  • Column ID: 3.0 mm
  • Hardware Material: Stainless Steel
  • Hardware Type: Analytical Column
  • Application: Peptides & Proteins
  • USP Code: L26
  • Temperature Range: 80 °C
  • pH Range: 2.5-8
  • Particle: Silica
  • Particle Shape: Spherical
  • Particle Type: Fully Porous
  • Surface Area: 100 m2/g
  • Bonding Type: monomeric
  • Package Size: 1

Questions about the article Questions about the article To the product details To the product details

Price:
EUR 1,064.00

Will be procured as soon as possible

RT-9512532 Viva C4 300Å 5µm, 2.1 x 30mm, ea.
EUR 569.00

Will be procured as soon as possible

Restek Viva C4 LC Columns
  • Product Number: RT-9512532
  • Manufacturer: Restek
  • Brand: Viva
  • Selectivity: C4
  • Particle Size: 5.0 µm
  • Pore Volume: 0.82 mL/g
  • Pore Size: 300 Å
  • Carbon Load: 3.5 %
  • Endcapping: yes
  • Column Length: 30 mm
  • Column ID: 2.1 mm
  • Hardware Material: Stainless Steel
  • Hardware Type: Analytical Column
  • Application: Peptides & Proteins
  • USP Code: L26
  • Temperature Range: 80 °C
  • pH Range: 2.5-8
  • Particle: Silica
  • Particle Shape: Spherical
  • Particle Type: Fully Porous
  • Surface Area: 100 m2/g
  • Bonding Type: monomeric
  • Package Size: 1

Questions about the article Questions about the article To the product details To the product details

Price:
EUR 569.00

Will be procured as soon as possible

RT-9512532-700 Viva C4 300Å 5µm, 2.1 x 30mm (Trident), ea. Price on request
Restek Viva C4 LC Columns
  • Product Number: RT-9512532-700
  • Manufacturer: Restek
  • Brand: Viva
  • Selectivity: C4
  • Particle Size: 5.0 µm
  • Pore Volume: 0.82 mL/g
  • Pore Size: 300 Å
  • Carbon Load: 3.5 %
  • Endcapping: yes
  • Column Length: 30 mm
  • Column ID: 2.1 mm
  • Hardware Material: Stainless Steel
  • Hardware Type: Analytical Column
  • Application: Peptides & Proteins
  • USP Code: L26
  • Temperature Range: 80 °C
  • pH Range: 2.5-8
  • Particle: Silica
  • Particle Shape: Spherical
  • Particle Type: Fully Porous
  • Surface Area: 100 m2/g
  • Bonding Type: monomeric
  • Package Size: 1

Questions about the article Questions about the article To the product details To the product details

Price on request
RT-9512535 Viva C4 300Å 5µm, 4.6 x 30mm, ea.
EUR 569.00

Will be procured as soon as possible

Restek Viva C4 LC Columns
  • Product Number: RT-9512535
  • Manufacturer: Restek
  • Brand: Viva
  • Selectivity: C4
  • Particle Size: 5.0 µm
  • Pore Volume: 0.82 mL/g
  • Pore Size: 300 Å
  • Carbon Load: 3.5 %
  • Endcapping: yes
  • Column Length: 30 mm
  • Column ID: 4.6 mm
  • Hardware Material: Stainless Steel
  • Hardware Type: Analytical Column
  • Application: Peptides & Proteins
  • USP Code: L26
  • Temperature Range: 80 °C
  • pH Range: 2.5-8
  • Particle: Silica
  • Particle Shape: Spherical
  • Particle Type: Fully Porous
  • Surface Area: 100 m2/g
  • Bonding Type: monomeric
  • Package Size: 1

Questions about the article Questions about the article To the product details To the product details

Price:
EUR 569.00

Will be procured as soon as possible

RT-9512535-700 Viva C4 300Å 5µm, 4.6 x 30mm (Trident), ea. Price on request
Restek Viva C4 LC Columns
  • Product Number: RT-9512535-700
  • Manufacturer: Restek
  • Brand: Viva
  • Selectivity: C4
  • Particle Size: 5.0 µm
  • Pore Volume: 0.82 mL/g
  • Pore Size: 300 Å
  • Carbon Load: 3.5 %
  • Endcapping: yes
  • Column Length: 30 mm
  • Column ID: 4.6 mm
  • Hardware Material: Stainless Steel
  • Hardware Type: Analytical Column
  • Application: Peptides & Proteins
  • USP Code: L26
  • Temperature Range: 80 °C
  • pH Range: 2.5-8
  • Particle: Silica
  • Particle Shape: Spherical
  • Particle Type: Fully Porous
  • Surface Area: 100 m2/g
  • Bonding Type: monomeric
  • Package Size: 1

Questions about the article Questions about the article To the product details To the product details

Price on request
RT-951253E Viva C4 300Å 5µm, 3.0 x 30mm, ea.
EUR 569.00

Will be procured as soon as possible

Restek Viva C4 LC Columns
  • Product Number: RT-951253E
  • Manufacturer: Restek
  • Brand: Viva
  • Selectivity: C4
  • Particle Size: 5.0 µm
  • Pore Volume: 0.82 mL/g
  • Pore Size: 300 Å
  • Carbon Load: 3.5 %
  • Endcapping: yes
  • Column Length: 30 mm
  • Column ID: 3.0 mm
  • Hardware Material: Stainless Steel
  • Hardware Type: Analytical Column
  • Application: Peptides & Proteins
  • USP Code: L26
  • Temperature Range: 80 °C
  • pH Range: 2.5-8
  • Particle: Silica
  • Particle Shape: Spherical
  • Particle Type: Fully Porous
  • Surface Area: 100 m2/g
  • Bonding Type: monomeric
  • Package Size: 1

Questions about the article Questions about the article To the product details To the product details

Price:
EUR 569.00

Will be procured as soon as possible

RT-9512551 Viva C4 300Å 5µm, 1.0 x 50mm, ea.
EUR 602.00

Will be procured as soon as possible

Restek Viva C4 LC Columns
  • Product Number: RT-9512551
  • Manufacturer: Restek
  • Brand: Viva
  • Selectivity: C4
  • Particle Size: 5.0 µm
  • Pore Volume: 0.82 mL/g
  • Pore Size: 300 Å
  • Carbon Load: 3.5 %
  • Endcapping: yes
  • Column Length: 50 mm
  • Column ID: 1.0 mm
  • Hardware Material: Stainless Steel
  • Hardware Type: Analytical Column
  • Application: Peptides & Proteins
  • USP Code: L26
  • Temperature Range: 80 °C
  • pH Range: 2.5-8
  • Particle: Silica
  • Particle Shape: Spherical
  • Particle Type: Fully Porous
  • Surface Area: 100 m2/g
  • Bonding Type: monomeric
  • Package Size: 1

Questions about the article Questions about the article To the product details To the product details

Price:
EUR 602.00

Will be procured as soon as possible

RT-9512552 Viva C4 300Å 5µm, 2.1 x 50mm, ea.
EUR 569.00

Will be procured as soon as possible

Restek Viva C4 LC Columns
  • Product Number: RT-9512552
  • Manufacturer: Restek
  • Brand: Viva
  • Selectivity: C4
  • Particle Size: 5.0 µm
  • Pore Volume: 0.82 mL/g
  • Pore Size: 300 Å
  • Carbon Load: 3.5 %
  • Endcapping: yes
  • Column Length: 50 mm
  • Column ID: 2.1 mm
  • Hardware Material: Stainless Steel
  • Hardware Type: Analytical Column
  • Application: Peptides & Proteins
  • USP Code: L26
  • Temperature Range: 80 °C
  • pH Range: 2.5-8
  • Particle: Silica
  • Particle Shape: Spherical
  • Particle Type: Fully Porous
  • Surface Area: 100 m2/g
  • Bonding Type: monomeric
  • Package Size: 1

Questions about the article Questions about the article To the product details To the product details

Price:
EUR 569.00

Will be procured as soon as possible

RT-9512552-700 Viva C4 300Å 5µm, 2.1 x 50mm (Trident), ea. Price on request
Restek Viva C4 LC Columns
  • Product Number: RT-9512552-700
  • Manufacturer: Restek
  • Brand: Viva
  • Selectivity: C4
  • Particle Size: 5.0 µm
  • Pore Volume: 0.82 mL/g
  • Pore Size: 300 Å
  • Carbon Load: 3.5 %
  • Endcapping: yes
  • Column Length: 50 mm
  • Column ID: 2.1 mm
  • Hardware Material: Stainless Steel
  • Hardware Type: Analytical Column
  • Application: Peptides & Proteins
  • USP Code: L26
  • Temperature Range: 80 °C
  • pH Range: 2.5-8
  • Particle: Silica
  • Particle Shape: Spherical
  • Particle Type: Fully Porous
  • Surface Area: 100 m2/g
  • Bonding Type: monomeric
  • Package Size: 1

Questions about the article Questions about the article To the product details To the product details

Price on request
RT-9512555 Viva C4 300Å 5µm, 4.6 x 50mm, ea.
EUR 580.00

Will be procured as soon as possible

Restek Viva C4 LC Columns
  • Product Number: RT-9512555
  • Manufacturer: Restek
  • Brand: Viva
  • Selectivity: C4
  • Particle Size: 5.0 µm
  • Pore Volume: 0.82 mL/g
  • Pore Size: 300 Å
  • Carbon Load: 3.5 %
  • Endcapping: yes
  • Column Length: 50 mm
  • Column ID: 4.6 mm
  • Hardware Material: Stainless Steel
  • Hardware Type: Analytical Column
  • Application: Peptides & Proteins
  • USP Code: L26
  • Temperature Range: 80 °C
  • pH Range: 2.5-8
  • Particle: Silica
  • Particle Shape: Spherical
  • Particle Type: Fully Porous
  • Surface Area: 100 m2/g
  • Bonding Type: monomeric
  • Package Size: 1

Questions about the article Questions about the article To the product details To the product details

Price:
EUR 580.00

Will be procured as soon as possible

RT-9512555-700 Viva C4 300Å 5µm, 4.6 x 50mm (Trident), ea. Price on request
Restek Viva C4 LC Columns
  • Product Number: RT-9512555-700
  • Manufacturer: Restek
  • Brand: Viva
  • Selectivity: C4
  • Particle Size: 5.0 µm
  • Pore Volume: 0.82 mL/g
  • Pore Size: 300 Å
  • Carbon Load: 3.5 %
  • Endcapping: yes
  • Column Length: 50 mm
  • Column ID: 4.6 mm
  • Hardware Material: Stainless Steel
  • Hardware Type: Analytical Column
  • Application: Peptides & Proteins
  • USP Code: L26
  • Temperature Range: 80 °C
  • pH Range: 2.5-8
  • Particle: Silica
  • Particle Shape: Spherical
  • Particle Type: Fully Porous
  • Surface Area: 100 m2/g
  • Bonding Type: monomeric
  • Package Size: 1

Questions about the article Questions about the article To the product details To the product details

Price on request
RT-951255E Viva C4 300Å 5µm, 3.0 x 50mm, ea.
EUR 569.00

Will be procured as soon as possible

Restek Viva C4 LC Columns
  • Product Number: RT-951255E
  • Manufacturer: Restek
  • Brand: Viva
  • Selectivity: C4
  • Particle Size: 5.0 µm
  • Pore Volume: 0.82 mL/g
  • Pore Size: 300 Å
  • Carbon Load: 3.5 %
  • Endcapping: yes
  • Column Length: 50 mm
  • Column ID: 3.0 mm
  • Hardware Material: Stainless Steel
  • Hardware Type: Analytical Column
  • Application: Peptides & Proteins
  • USP Code: L26
  • Temperature Range: 80 °C
  • pH Range: 2.5-8
  • Particle: Silica
  • Particle Shape: Spherical
  • Particle Type: Fully Porous
  • Surface Area: 100 m2/g
  • Bonding Type: monomeric
  • Package Size: 1

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Price:
EUR 569.00

Will be procured as soon as possible

RT-9512561 Viva C4 300Å 5µm, 1.0 x 150mm, ea.
EUR 715.00

Will be procured as soon as possible

Restek Viva C4 LC Columns
  • Product Number: RT-9512561
  • Manufacturer: Restek
  • Brand: Viva
  • Selectivity: C4
  • Particle Size: 5.0 µm
  • Pore Volume: 0.82 mL/g
  • Pore Size: 300 Å
  • Carbon Load: 3.5 %
  • Endcapping: yes
  • Column Length: 150 mm
  • Column ID: 1.0 mm
  • Hardware Material: Stainless Steel
  • Hardware Type: Analytical Column
  • Application: Peptides & Proteins
  • USP Code: L26
  • Temperature Range: 80 °C
  • pH Range: 2.5-8
  • Particle: Silica
  • Particle Shape: Spherical
  • Particle Type: Fully Porous
  • Surface Area: 100 m2/g
  • Bonding Type: monomeric
  • Package Size: 1

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Price:
EUR 715.00

Will be procured as soon as possible

Advanced 300 Å Wide Pore Silica Technology

Efficient separation of proteins and peptides requires chromatographic media with pores that are readily accessible to large molecules. While many wide pore silica materials are specified with an average pore size of 300 Å, they often exhibit a broad pore size distribution, leaving a considerable portion of the internal surface inaccessible to larger analytes.

Restek developed the Viva™ Wide Pore silica using a tightly controlled manufacturing process that produces a narrow pore size distribution centered around 300 Å. This design maximizes the available surface area within the optimal pore size range, allowing proteins and peptides to interact more effectively with the stationary phase. The result is improved retention, enhanced mass transfer, higher efficiency, and excellent chromatographic resolution for biomolecule separations.

C4 Selectivity for Large Biomolecules

The Viva™ C4 stationary phase is based on a monomeric butylsilane chemistry (USP L26) that provides lower hydrophobic retention than C8 or C18 phases. This reduced retention is especially advantageous for intact proteins, therapeutic proteins, and other large biomolecules, enabling efficient elution while maintaining excellent separation performance.

The end-capped stationary phase minimizes secondary interactions with residual silanol groups, resulting in improved peak symmetry and high analyte recovery.

Typical applications include:

  • Intact protein analysis
  • Peptide characterization
  • Therapeutic protein analysis
  • Monoclonal antibody research
  • Recombinant protein analysis
  • Protein purity assessment
  • Protein degradation studies
  • Biopharmaceutical quality control
  • LC-MS method development

Key Features and Benefits

  • Optimized 300 Å wide pore silica for proteins and peptides
  • Narrow pore size distribution for maximum accessible surface area
  • Monomeric C4 stationary phase (USP L26)
  • Lower hydrophobic retention than C8 and C18 phases
  • High recovery of intact proteins and large biomolecules
  • Excellent peak symmetry through end-capped surface chemistry
  • High-purity, spherical silica particles
  • Outstanding batch-to-batch reproducibility
  • Compatible with HPLC, UHPLC, LC-UV, and LC-MS workflows
  • Robust performance for routine and research laboratories

Consistent Performance Through Integrated Manufacturing

Every Restek Viva™ column is manufactured entirely by Restek, from the production of the base silica through stationary phase bonding and final column packing. This vertically integrated manufacturing process ensures exceptional consistency between production batches, providing reliable retention times, reproducible selectivity, and long column lifetimes.

The optimized wide pore silica and carefully controlled C4 bonding chemistry make Viva™ C4 columns a dependable solution for laboratories performing routine protein analysis as well as complex biomolecular method development.

Typical Applications

Restek Viva™ Wide Pore 300 Å C4 columns are ideal for:

  • Intact protein separations
  • Therapeutic protein characterization
  • Monoclonal antibody analysis
  • Recombinant protein quality control
  • Protein digest analysis
  • Peptide purification
  • Proteomics research
  • Biomarker discovery
  • Biopharmaceutical method development
  • LC-MS analysis of proteins and peptides

The Ideal Solution for Large Biomolecule Analysis

The Restek Viva™ Wide Pore 300 Å C4 HPLC column combines optimized pore accessibility with moderate hydrophobic C4 selectivity to deliver reliable, high-resolution separations of proteins and peptides. Its reduced retention compared with C18 and C8 phases makes it particularly well suited for intact proteins and other high molecular weight biomolecules, while the advanced silica technology ensures excellent peak shape, reproducibility, and long-term performance.

Whether used for routine quality control or advanced biopharmaceutical research, Viva™ C4 columns provide the robustness, consistency, and chromatographic efficiency required for today's demanding bioanalytical laboratories.

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