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Viva C18 300Å Series
Restek Viva LC Columns C18 Header Picture

Restek Viva™ Wide Pore 300 Å C18 HPLC Columns

High-Performance C18 Columns for Protein and Peptide Analysis

The Restek Viva™ Wide Pore 300 Å C18 HPLC column series has been specifically engineered for the reversed-phase separation of proteins, peptides, and other high molecular weight biomolecules. By combining a high-purity silica support with an optimized wide pore structure and a robust monomeric C18 bonded phase, Viva C18 columns deliver outstanding chromatographic performance for both routine bioanalytical applications and advanced method development.

Designed for LC-UV and LC-MS workflows, Viva C18 columns provide excellent retention, high recovery, and exceptional reproducibility for peptide mapping, intact protein analysis, and biopharmaceutical characterization.

This unique design delivers enhanced retention, improved resolution, and exceptional chromatographic performance for biomolecule analysis, making Viva columns an ideal choice for biopharmaceutical research, proteomics, peptide characterization, and LC-MS applications.

Technical Specifications

Property Specification
Stationary Phase Monomeric C18 (USP L1)
Pore Size 300 Å
Particle Sizes 3 µm and 5 µm
Surface Area 100 m²/g
Carbon Load 9%
End-Capped Yes
pH Range 2.5–8.0

Maximum Operating Temperature 80 °C

Find more information in the Restek Viva LC Columns Brochure.

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59 Products
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Product Number Description Price Specifications
RT-9514311 Viva C18 300Å 3µm, 1.0 x 100mm, ea.
EUR 780.00

Will be procured as soon as possible

Restek Viva C18 LC Columns
  • Product Number: RT-9514311
  • Manufacturer: Restek
  • Brand: Viva
  • Selectivity: C18
  • Particle Size: 3.0 µm
  • Pore Volume: 0.84 mL/g
  • Pore Size: 300 Å
  • Carbon Load: 9.0 %
  • Endcapping: yes
  • Column Length: 100 mm
  • Column ID: 1.0 mm
  • Hardware Material: Stainless Steel
  • Hardware Type: Analytical Column
  • Application: Peptides & Proteins
  • USP Code: L1
  • Temperature Range: 80 °C
  • pH Range: 2.5-8
  • Particle: Silica
  • Particle Shape: Spherical
  • Particle Type: Fully Porous
  • Surface Area: 100 m2/g
  • Bonding Type: monomeric
  • Package Size: 1

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Price:
EUR 780.00

Will be procured as soon as possible

RT-9514312 Viva C18 300Å 3µm, 2.1 x 100mm, ea.
EUR 741.00

Will be procured as soon as possible

Restek Viva C18 LC Columns
  • Product Number: RT-9514312
  • Manufacturer: Restek
  • Brand: Viva
  • Selectivity: C18
  • Particle Size: 3.0 µm
  • Pore Volume: 0.84 mL/g
  • Pore Size: 300 Å
  • Carbon Load: 9.0 %
  • Endcapping: yes
  • Column Length: 100 mm
  • Column ID: 2.1 mm
  • Hardware Material: Stainless Steel
  • Hardware Type: Analytical Column
  • Application: Peptides & Proteins
  • USP Code: L1
  • Temperature Range: 80 °C
  • pH Range: 2.5-8
  • Particle: Silica
  • Particle Shape: Spherical
  • Particle Type: Fully Porous
  • Surface Area: 100 m2/g
  • Bonding Type: monomeric
  • Package Size: 1

Questions about the article Questions about the article To the product details To the product details

Price:
EUR 741.00

Will be procured as soon as possible

RT-9514312-700 Viva C18 300Å 3µm, 2.1 x 100mm (Trident), ea.
EUR 775.00

Will be procured as soon as possible

Restek Viva C18 LC Columns
  • Product Number: RT-9514312-700
  • Manufacturer: Restek
  • Brand: Viva
  • Selectivity: C18
  • Particle Size: 3.0 µm
  • Pore Volume: 0.84 mL/g
  • Pore Size: 300 Å
  • Carbon Load: 9.0 %
  • Endcapping: yes
  • Column Length: 100 mm
  • Column ID: 2.1 mm
  • Hardware Material: Stainless Steel
  • Hardware Type: Analytical Column
  • Application: Peptides & Proteins
  • USP Code: L1
  • Temperature Range: 80 °C
  • pH Range: 2.5-8
  • Particle: Silica
  • Particle Shape: Spherical
  • Particle Type: Fully Porous
  • Surface Area: 100 m2/g
  • Bonding Type: monomeric
  • Package Size: 1

Questions about the article Questions about the article To the product details To the product details

Price:
EUR 775.00

Will be procured as soon as possible

RT-9514315 Viva C18 300Å 3µm, 4.6 x 100mm, ea.
EUR 1,088.00

Will be procured as soon as possible

Restek Viva C18 LC Columns
  • Product Number: RT-9514315
  • Manufacturer: Restek
  • Brand: Viva
  • Selectivity: C18
  • Particle Size: 3.0 µm
  • Pore Volume: 0.84 mL/g
  • Pore Size: 300 Å
  • Carbon Load: 9.0 %
  • Endcapping: yes
  • Column Length: 100 mm
  • Column ID: 4.6 mm
  • Hardware Material: Stainless Steel
  • Hardware Type: Analytical Column
  • Application: Peptides & Proteins
  • USP Code: L1
  • Temperature Range: 80 °C
  • pH Range: 2.5-8
  • Particle: Silica
  • Particle Shape: Spherical
  • Particle Type: Fully Porous
  • Surface Area: 100 m2/g
  • Bonding Type: monomeric
  • Package Size: 1

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Price:
EUR 1,088.00

Will be procured as soon as possible

RT-9514315-700 Viva C18 300Å 3µm, 4.6 x 100mm (Trident), ea. Price on request
Restek Viva C18 LC Columns
  • Product Number: RT-9514315-700
  • Manufacturer: Restek
  • Brand: Viva
  • Selectivity: C18
  • Particle Size: 3.0 µm
  • Pore Volume: 0.84 mL/g
  • Pore Size: 300 Å
  • Carbon Load: 9.0 %
  • Endcapping: yes
  • Column Length: 100 mm
  • Column ID: 4.6 mm
  • Hardware Material: Stainless Steel
  • Hardware Type: Analytical Column
  • Application: Peptides & Proteins
  • USP Code: L1
  • Temperature Range: 80 °C
  • pH Range: 2.5-8
  • Particle: Silica
  • Particle Shape: Spherical
  • Particle Type: Fully Porous
  • Surface Area: 100 m2/g
  • Bonding Type: monomeric
  • Package Size: 1

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Price on request
RT-951431E Viva C18 300Å 3µm, 3.0 x 100mm, ea.
EUR 907.00

Will be procured as soon as possible

Restek Viva C18 LC Columns
  • Product Number: RT-951431E
  • Manufacturer: Restek
  • Brand: Viva
  • Selectivity: C18
  • Particle Size: 3.0 µm
  • Pore Volume: 0.84 mL/g
  • Pore Size: 300 Å
  • Carbon Load: 9.0 %
  • Endcapping: yes
  • Column Length: 100 mm
  • Column ID: 3.0 mm
  • Hardware Material: Stainless Steel
  • Hardware Type: Analytical Column
  • Application: Peptides & Proteins
  • USP Code: L1
  • Temperature Range: 80 °C
  • pH Range: 2.5-8
  • Particle: Silica
  • Particle Shape: Spherical
  • Particle Type: Fully Porous
  • Surface Area: 100 m2/g
  • Bonding Type: monomeric
  • Package Size: 1

Questions about the article Questions about the article To the product details To the product details

Price:
EUR 907.00

Will be procured as soon as possible

RT-9514332 Viva C18 300Å 3µm, 2.1 x 30mm, ea.
EUR 701.00

Will be procured as soon as possible

Restek Viva C18 LC Columns
  • Product Number: RT-9514332
  • Manufacturer: Restek
  • Brand: Viva
  • Selectivity: C18
  • Particle Size: 3.0 µm
  • Pore Volume: 0.84 mL/g
  • Pore Size: 300 Å
  • Carbon Load: 9.0 %
  • Endcapping: yes
  • Column Length: 30 mm
  • Column ID: 2.1 mm
  • Hardware Material: Stainless Steel
  • Hardware Type: Analytical Column
  • Application: Peptides & Proteins
  • USP Code: L1
  • Temperature Range: 80 °C
  • pH Range: 2.5-8
  • Particle: Silica
  • Particle Shape: Spherical
  • Particle Type: Fully Porous
  • Surface Area: 100 m2/g
  • Bonding Type: monomeric
  • Package Size: 1

Questions about the article Questions about the article To the product details To the product details

Price:
EUR 701.00

Will be procured as soon as possible

RT-9514332-700 Viva C18 300Å 3µm, 2.1 x 30mm (Trident), ea. Price on request
Restek Viva C18 LC Columns
  • Product Number: RT-9514332-700
  • Manufacturer: Restek
  • Brand: Viva
  • Selectivity: C18
  • Particle Size: 3.0 µm
  • Pore Volume: 0.84 mL/g
  • Pore Size: 300 Å
  • Carbon Load: 9.0 %
  • Endcapping: yes
  • Column Length: 30 mm
  • Column ID: 2.1 mm
  • Hardware Material: Stainless Steel
  • Hardware Type: Analytical Column
  • Application: Peptides & Proteins
  • USP Code: L1
  • Temperature Range: 80 °C
  • pH Range: 2.5-8
  • Particle: Silica
  • Particle Shape: Spherical
  • Particle Type: Fully Porous
  • Surface Area: 100 m2/g
  • Bonding Type: monomeric
  • Package Size: 1

Questions about the article Questions about the article To the product details To the product details

Price on request
RT-9514335 Viva C18 300Å 3µm, 4.6 x 30mm, ea.
EUR 741.00

Will be procured as soon as possible

Restek Viva C18 LC Columns
  • Product Number: RT-9514335
  • Manufacturer: Restek
  • Brand: Viva
  • Selectivity: C18
  • Particle Size: 3.0 µm
  • Pore Volume: 0.84 mL/g
  • Pore Size: 300 Å
  • Carbon Load: 9.0 %
  • Endcapping: yes
  • Column Length: 30 mm
  • Column ID: 4.6 mm
  • Hardware Material: Stainless Steel
  • Hardware Type: Analytical Column
  • Application: Peptides & Proteins
  • USP Code: L1
  • Temperature Range: 80 °C
  • pH Range: 2.5-8
  • Particle: Silica
  • Particle Shape: Spherical
  • Particle Type: Fully Porous
  • Surface Area: 100 m2/g
  • Bonding Type: monomeric
  • Package Size: 1

Questions about the article Questions about the article To the product details To the product details

Price:
EUR 741.00

Will be procured as soon as possible

RT-9514335-700 Viva C18 300Å 3µm, 4.6 x 30mm (Trident), ea. Price on request
Restek Viva C18 LC Columns
  • Product Number: RT-9514335-700
  • Manufacturer: Restek
  • Brand: Viva
  • Selectivity: C18
  • Particle Size: 3.0 µm
  • Pore Volume: 0.84 mL/g
  • Pore Size: 300 Å
  • Carbon Load: 9.0 %
  • Endcapping: yes
  • Column Length: 30 mm
  • Column ID: 4.6 mm
  • Hardware Material: Stainless Steel
  • Hardware Type: Analytical Column
  • Application: Peptides & Proteins
  • USP Code: L1
  • Temperature Range: 80 °C
  • pH Range: 2.5-8
  • Particle: Silica
  • Particle Shape: Spherical
  • Particle Type: Fully Porous
  • Surface Area: 100 m2/g
  • Bonding Type: monomeric
  • Package Size: 1

Questions about the article Questions about the article To the product details To the product details

Price on request
RT-951433E Viva C18 300Å 3µm, 3.0 x 30mm, ea.
EUR 701.00

Will be procured as soon as possible

Restek Viva C18 LC Columns
  • Product Number: RT-951433E
  • Manufacturer: Restek
  • Brand: Viva
  • Selectivity: C18
  • Particle Size: 3.0 µm
  • Pore Volume: 0.84 mL/g
  • Pore Size: 300 Å
  • Carbon Load: 9.0 %
  • Endcapping: yes
  • Column Length: 30 mm
  • Column ID: 3.0 mm
  • Hardware Material: Stainless Steel
  • Hardware Type: Analytical Column
  • Application: Peptides & Proteins
  • USP Code: L1
  • Temperature Range: 80 °C
  • pH Range: 2.5-8
  • Particle: Silica
  • Particle Shape: Spherical
  • Particle Type: Fully Porous
  • Surface Area: 100 m2/g
  • Bonding Type: monomeric
  • Package Size: 1

Questions about the article Questions about the article To the product details To the product details

Price:
EUR 701.00

Will be procured as soon as possible

RT-9514351 Viva C18 300Å 3µm, 1.0 x 50mm, ea.
EUR 727.00

Will be procured as soon as possible

Restek Viva C18 LC Columns
  • Product Number: RT-9514351
  • Manufacturer: Restek
  • Brand: Viva
  • Selectivity: C18
  • Particle Size: 3.0 µm
  • Pore Volume: 0.84 mL/g
  • Pore Size: 300 Å
  • Carbon Load: 9.0 %
  • Endcapping: yes
  • Column Length: 50 mm
  • Column ID: 1.0 mm
  • Hardware Material: Stainless Steel
  • Hardware Type: Analytical Column
  • Application: Peptides & Proteins
  • USP Code: L1
  • Temperature Range: 80 °C
  • pH Range: 2.5-8
  • Particle: Silica
  • Particle Shape: Spherical
  • Particle Type: Fully Porous
  • Surface Area: 100 m2/g
  • Bonding Type: monomeric
  • Package Size: 1

Questions about the article Questions about the article To the product details To the product details

Price:
EUR 727.00

Will be procured as soon as possible

RT-9514352 Viva C18 300Å 3µm, 2.1 x 50mm, ea.
EUR 701.00

Will be procured as soon as possible

Restek Viva C18 LC Columns
  • Product Number: RT-9514352
  • Manufacturer: Restek
  • Brand: Viva
  • Selectivity: C18
  • Particle Size: 3.0 µm
  • Pore Volume: 0.84 mL/g
  • Pore Size: 300 Å
  • Carbon Load: 9.0 %
  • Endcapping: yes
  • Column Length: 50 mm
  • Column ID: 2.1 mm
  • Hardware Material: Stainless Steel
  • Hardware Type: Analytical Column
  • Application: Peptides & Proteins
  • USP Code: L1
  • Temperature Range: 80 °C
  • pH Range: 2.5-8
  • Particle: Silica
  • Particle Shape: Spherical
  • Particle Type: Fully Porous
  • Surface Area: 100 m2/g
  • Bonding Type: monomeric
  • Package Size: 1

Questions about the article Questions about the article To the product details To the product details

Price:
EUR 701.00

Will be procured as soon as possible

RT-9514352-700 Viva C18 300Å 3µm, 2.1 x 50mm (Trident), ea. Price on request
Restek Viva C18 LC Columns
  • Product Number: RT-9514352-700
  • Manufacturer: Restek
  • Brand: Viva
  • Selectivity: C18
  • Particle Size: 3.0 µm
  • Pore Volume: 0.84 mL/g
  • Pore Size: 300 Å
  • Carbon Load: 9.0 %
  • Endcapping: yes
  • Column Length: 50 mm
  • Column ID: 2.1 mm
  • Hardware Material: Stainless Steel
  • Hardware Type: Analytical Column
  • Application: Peptides & Proteins
  • USP Code: L1
  • Temperature Range: 80 °C
  • pH Range: 2.5-8
  • Particle: Silica
  • Particle Shape: Spherical
  • Particle Type: Fully Porous
  • Surface Area: 100 m2/g
  • Bonding Type: monomeric
  • Package Size: 1

Questions about the article Questions about the article To the product details To the product details

Price on request
RT-9514355 Viva C18 300Å 3µm, 4.6 x 50mm, ea.
EUR 876.00

Will be procured as soon as possible

Restek Viva C18 LC Columns
  • Product Number: RT-9514355
  • Manufacturer: Restek
  • Brand: Viva
  • Selectivity: C18
  • Particle Size: 3.0 µm
  • Pore Volume: 0.84 mL/g
  • Pore Size: 300 Å
  • Carbon Load: 9.0 %
  • Endcapping: yes
  • Column Length: 50 mm
  • Column ID: 4.6 mm
  • Hardware Material: Stainless Steel
  • Hardware Type: Analytical Column
  • Application: Peptides & Proteins
  • USP Code: L1
  • Temperature Range: 80 °C
  • pH Range: 2.5-8
  • Particle: Silica
  • Particle Shape: Spherical
  • Particle Type: Fully Porous
  • Surface Area: 100 m2/g
  • Bonding Type: monomeric
  • Package Size: 1

Questions about the article Questions about the article To the product details To the product details

Price:
EUR 876.00

Will be procured as soon as possible

RT-9514355-700 Viva C18 300Å 3µm, 4.6 x 50mm (Trident), ea. Price on request
Restek Viva C18 LC Columns
  • Product Number: RT-9514355-700
  • Manufacturer: Restek
  • Brand: Viva
  • Selectivity: C18
  • Particle Size: 3.0 µm
  • Pore Volume: 0.84 mL/g
  • Pore Size: 300 Å
  • Carbon Load: 9.0 %
  • Endcapping: yes
  • Column Length: 50 mm
  • Column ID: 4.6 mm
  • Hardware Material: Stainless Steel
  • Hardware Type: Analytical Column
  • Application: Peptides & Proteins
  • USP Code: L1
  • Temperature Range: 80 °C
  • pH Range: 2.5-8
  • Particle: Silica
  • Particle Shape: Spherical
  • Particle Type: Fully Porous
  • Surface Area: 100 m2/g
  • Bonding Type: monomeric
  • Package Size: 1

Questions about the article Questions about the article To the product details To the product details

Price on request
RT-951435E Viva C18 300Å 3µm, 3.0 x 50mm, ea.
EUR 701.00

Will be procured as soon as possible

Restek Viva C18 LC Columns
  • Product Number: RT-951435E
  • Manufacturer: Restek
  • Brand: Viva
  • Selectivity: C18
  • Particle Size: 3.0 µm
  • Pore Volume: 0.84 mL/g
  • Pore Size: 300 Å
  • Carbon Load: 9.0 %
  • Endcapping: yes
  • Column Length: 50 mm
  • Column ID: 3.0 mm
  • Hardware Material: Stainless Steel
  • Hardware Type: Analytical Column
  • Application: Peptides & Proteins
  • USP Code: L1
  • Temperature Range: 80 °C
  • pH Range: 2.5-8
  • Particle: Silica
  • Particle Shape: Spherical
  • Particle Type: Fully Porous
  • Surface Area: 100 m2/g
  • Bonding Type: monomeric
  • Package Size: 1

Questions about the article Questions about the article To the product details To the product details

Price:
EUR 701.00

Will be procured as soon as possible

RT-9514361 Viva C18 300Å 3µm, 1.0 x 150mm, ea.
EUR 837.00

Will be procured as soon as possible

Restek Viva C18 LC Columns
  • Product Number: RT-9514361
  • Manufacturer: Restek
  • Brand: Viva
  • Selectivity: C18
  • Particle Size: 3.0 µm
  • Pore Volume: 0.84 mL/g
  • Pore Size: 300 Å
  • Carbon Load: 9.0 %
  • Endcapping: yes
  • Column Length: 150 mm
  • Column ID: 1.0 mm
  • Hardware Material: Stainless Steel
  • Hardware Type: Analytical Column
  • Application: Peptides & Proteins
  • USP Code: L1
  • Temperature Range: 80 °C
  • pH Range: 2.5-8
  • Particle: Silica
  • Particle Shape: Spherical
  • Particle Type: Fully Porous
  • Surface Area: 100 m2/g
  • Bonding Type: monomeric
  • Package Size: 1

Questions about the article Questions about the article To the product details To the product details

Price:
EUR 837.00

Will be procured as soon as possible

RT-9514362 Viva C18 300Å 3µm, 2.1 x 150mm, ea.
EUR 827.00

Will be procured as soon as possible

Restek Viva C18 LC Columns
  • Product Number: RT-9514362
  • Manufacturer: Restek
  • Brand: Viva
  • Selectivity: C18
  • Particle Size: 3.0 µm
  • Pore Volume: 0.84 mL/g
  • Pore Size: 300 Å
  • Carbon Load: 9.0 %
  • Endcapping: yes
  • Column Length: 150 mm
  • Column ID: 2.1 mm
  • Hardware Material: Stainless Steel
  • Hardware Type: Analytical Column
  • Application: Peptides & Proteins
  • USP Code: L1
  • Temperature Range: 80 °C
  • pH Range: 2.5-8
  • Particle: Silica
  • Particle Shape: Spherical
  • Particle Type: Fully Porous
  • Surface Area: 100 m2/g
  • Bonding Type: monomeric
  • Package Size: 1

Questions about the article Questions about the article To the product details To the product details

Price:
EUR 827.00

Will be procured as soon as possible

RT-9514362-700 Viva C18 300Å 3µm, 2.1 x 150mm (Trident), ea.
EUR 845.00

Will be procured as soon as possible

Restek Viva C18 LC Columns
  • Product Number: RT-9514362-700
  • Manufacturer: Restek
  • Brand: Viva
  • Selectivity: C18
  • Particle Size: 3.0 µm
  • Pore Volume: 0.84 mL/g
  • Pore Size: 300 Å
  • Carbon Load: 9.0 %
  • Endcapping: yes
  • Column Length: 150 mm
  • Column ID: 2.1 mm
  • Hardware Material: Stainless Steel
  • Hardware Type: Analytical Column
  • Application: Peptides & Proteins
  • USP Code: L1
  • Temperature Range: 80 °C
  • pH Range: 2.5-8
  • Particle: Silica
  • Particle Shape: Spherical
  • Particle Type: Fully Porous
  • Surface Area: 100 m2/g
  • Bonding Type: monomeric
  • Package Size: 1

Questions about the article Questions about the article To the product details To the product details

Price:
EUR 845.00

Will be procured as soon as possible

RT-9514365 Viva C18 300Å 3µm, 4.6 x 150mm, ea.
EUR 1,381.00

Will be procured as soon as possible

Restek Viva C18 LC Columns
  • Product Number: RT-9514365
  • Manufacturer: Restek
  • Brand: Viva
  • Selectivity: C18
  • Particle Size: 3.0 µm
  • Pore Volume: 0.84 mL/g
  • Pore Size: 300 Å
  • Carbon Load: 9.0 %
  • Endcapping: yes
  • Column Length: 150 mm
  • Column ID: 4.6 mm
  • Hardware Material: Stainless Steel
  • Hardware Type: Analytical Column
  • Application: Peptides & Proteins
  • USP Code: L1
  • Temperature Range: 80 °C
  • pH Range: 2.5-8
  • Particle: Silica
  • Particle Shape: Spherical
  • Particle Type: Fully Porous
  • Surface Area: 100 m2/g
  • Bonding Type: monomeric
  • Package Size: 1

Questions about the article Questions about the article To the product details To the product details

Price:
EUR 1,381.00

Will be procured as soon as possible

RT-9514365-700 Viva C18 300Å 3µm, 4.6 x 150mm (Trident), ea. Price on request
Restek Viva C18 LC Columns
  • Product Number: RT-9514365-700
  • Manufacturer: Restek
  • Brand: Viva
  • Selectivity: C18
  • Particle Size: 3.0 µm
  • Pore Volume: 0.84 mL/g
  • Pore Size: 300 Å
  • Carbon Load: 9.0 %
  • Endcapping: yes
  • Column Length: 150 mm
  • Column ID: 4.6 mm
  • Hardware Material: Stainless Steel
  • Hardware Type: Analytical Column
  • Application: Peptides & Proteins
  • USP Code: L1
  • Temperature Range: 80 °C
  • pH Range: 2.5-8
  • Particle: Silica
  • Particle Shape: Spherical
  • Particle Type: Fully Porous
  • Surface Area: 100 m2/g
  • Bonding Type: monomeric
  • Package Size: 1

Questions about the article Questions about the article To the product details To the product details

Price on request
RT-951436E Viva C18 300Å 3µm, 3.0 x 150mm, ea.
EUR 1,130.00

Will be procured as soon as possible

Restek Viva C18 LC Columns
  • Product Number: RT-951436E
  • Manufacturer: Restek
  • Brand: Viva
  • Selectivity: C18
  • Particle Size: 3.0 µm
  • Pore Volume: 0.84 mL/g
  • Pore Size: 300 Å
  • Carbon Load: 9.0 %
  • Endcapping: yes
  • Column Length: 150 mm
  • Column ID: 3.0 mm
  • Hardware Material: Stainless Steel
  • Hardware Type: Analytical Column
  • Application: Peptides & Proteins
  • USP Code: L1
  • Temperature Range: 80 °C
  • pH Range: 2.5-8
  • Particle: Silica
  • Particle Shape: Spherical
  • Particle Type: Fully Porous
  • Surface Area: 100 m2/g
  • Bonding Type: monomeric
  • Package Size: 1

Questions about the article Questions about the article To the product details To the product details

Price:
EUR 1,130.00

Will be procured as soon as possible

RT-951450210 Viva C18 300Å 5µm, 4.0 x 10mm Guard Cartridge (Trident), pk.3
EUR 306.00

Will be procured as soon as possible

Restek Viva C18 Guard Cartridges
  • Product Number: RT-951450210
  • Manufacturer: Restek
  • Brand: Viva
  • Selectivity: C18
  • Particle Size: 5.0 µm
  • Pore Volume: 0.82 mL/g
  • Pore Size: 300 Å
  • Carbon Load: 9.0 %
  • Endcapping: yes
  • Column Length: 10 mm
  • Column ID: 4.0 mm
  • Hardware Material: Stainless Steel
  • Hardware Type: Guard Cartridge
  • Application: Peptides & Proteins
  • USP Code: L1
  • Temperature Range: 80 °C
  • pH Range: 2.5-8
  • Particle: Silica
  • Particle Shape: Spherical
  • Particle Type: Fully Porous
  • Surface Area: 100 m2/g
  • Bonding Type: monomeric
  • Accessories: Guard Cartridge
  • Package Size: 3

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Price:
EUR 306.00

Will be procured as soon as possible

RT-951450212 Viva C18 300Å 5µm, 2.1 x 10mm Guard Cartridge (Trident), pk.3
EUR 254.00

Will be procured as soon as possible

Restek Viva C18 Guard Cartridges
  • Product Number: RT-951450212
  • Manufacturer: Restek
  • Brand: Viva
  • Selectivity: C18
  • Particle Size: 5.0 µm
  • Pore Volume: 0.82 mL/g
  • Pore Size: 300 Å
  • Carbon Load: 9.0 %
  • Endcapping: yes
  • Column Length: 10 mm
  • Column ID: 2.1 mm
  • Hardware Material: Stainless Steel
  • Hardware Type: Guard Cartridge
  • Application: Peptides & Proteins
  • USP Code: L1
  • Temperature Range: 80 °C
  • pH Range: 2.5-8
  • Particle: Silica
  • Particle Shape: Spherical
  • Particle Type: Fully Porous
  • Surface Area: 100 m2/g
  • Bonding Type: monomeric
  • Accessories: Guard Cartridge
  • Package Size: 3

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Price:
EUR 254.00

Will be procured as soon as possible

Optimized 300 Å Wide Pore Silica

Large biomolecules require sufficient pore accessibility to fully interact with the stationary phase. While many conventional wide pore silica materials are specified with an average pore size of 300 Å, they often contain a broad pore size distribution, reducing the effective surface area available to proteins and peptides.

Restek Viva™ Wide Pore columns are manufactured using a carefully controlled silica production process that creates a narrow pore size distribution centered around 300 Å. This design provides greater accessible surface area for large molecules, resulting in improved analyte retention, enhanced mass transfer, and superior chromatographic efficiency.

General-Purpose C18 Selectivity for Biomolecules

The Viva™ C18 stationary phase is a conventional monomeric octadecylsilane (USP L1) chemistry that offers balanced hydrophobic retention for a broad range of biomolecules. It is ideally suited for the separation of peptides, small proteins, recombinant proteins, and many therapeutic biomolecules.

Its versatile selectivity makes Viva C18 an excellent first-choice column for:

  • Peptide mapping
  • Protein purity testing
  • Intact protein analysis
  • Biopharmaceutical characterization
  • Enzymatic digest analysis
  • Proteomics research
  • LC-MS method development
  • Quality control laboratories

The end-capped stationary phase minimizes unwanted secondary interactions, promoting excellent peak symmetry and reproducible retention for both acidic and moderately basic analytes.

Key Features and Benefits

  • 300 Å wide pore silica optimized for proteins and peptides
  • Narrow pore size distribution for maximum accessible surface area
  • High-purity, base-deactivated silica
  • Monomeric C18 bonded phase (USP L1)
  • End-capped surface for improved peak shape
  • Excellent recovery of large biomolecules
  • High chromatographic efficiency
  • Outstanding lot-to-lot reproducibility
  • Compatible with LC-UV and LC-MS detection
  • Available in multiple column dimensions and 3 µm and 5 µm particle sizes for analytical HPLC and UHPLC applications.

Reliable Performance for Biopharmaceutical Analysis

Modern protein and peptide analysis demands chromatographic columns that combine high efficiency with exceptional robustness. Restek manufactures the complete Viva™ column—from silica synthesis through stationary phase bonding and final column packing—ensuring consistent quality and reproducible chromatographic performance from batch to batch.

This integrated manufacturing approach helps laboratories maintain reliable analytical methods throughout the lifetime of the column while simplifying method transfer between instruments and production lots.

Typical Applications

Restek Viva™ Wide Pore 300 Å C18 columns are widely used for:

  • Peptide mapping and characterization
  • Therapeutic protein analysis
  • Monoclonal antibody research
  • Recombinant protein purification
  • Biopharmaceutical quality control
  • Protein degradation studies
  • Biomarker research
  • Proteomics and bioanalytical LC-MS

The Ideal Choice for Large Molecule Separations

Whether you are developing new analytical methods or performing routine quality control, the Restek Viva™ Wide Pore 300 Å C18 HPLC column provides the performance required for reliable protein and peptide separations. Its optimized pore architecture, proven C18 selectivity, and excellent manufacturing consistency make it a dependable solution for modern bioanalytical laboratories seeking high resolution, reproducibility, and robust chromatographic performance.

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