Agilent AdvanceBio Peptide Mapping Columns

Agilent AdvanceBio Peptide Mapping columns are reversed-phase HPLC columns developed for high-resolution peptide mapping, protein identification and the determination of post-translational modifications. They support the separation of peptides generated by enzymatic protein digestion and can be used to identify amino acid substitutions or modifications within the primary protein sequence.

The columns use 2.7 µm superficially porous particles with a 120 Å pore size, providing suitable pore accessibility for peptides produced by enzymatic digestion of proteins. The 2.7 µm particle technology enables UHPLC-level performance on HPLC systems and provides high-resolution separations 2 to 3 times faster than fully porous HPLC columns. Combination of the chromatographic separation with mass spectrometry detection allows observed peptide peaks to be correlated with predicted protein fragments.

Key Features of AdvanceBio Peptide Mapping Columns

• Designed for high-resolution peptide mapping and protein characterization
  
• Suitable for identification of post-translational modifications and amino acid substitutions
  
• 2.7 µm superficially porous particles with 120 Å pore size for analysis of peptides
  
• Reversed-phase chromatography for peptides with different sizes and hydrophobicities
  
• Suitable for combination with mass spectrometry detection
  
• Each batch of AdvanceBio Peptide Mapping media is tested with a peptide mixture for suitability and reproducibility
  

For Research Use Only. Not for use in diagnostic procedures.

Use the product filters below to select the appropriate Agilent AdvanceBio Peptide Mapping column according to column dimensions and peptide analysis requirements.