Agilent AdvanceBio Peptide Plus Columns

Agilent AdvanceBio Peptide Plus columns are reversed-phase HPLC columns optimized for the separation and characterization of target peptides, peptide impurities and post-translational modifications. The columns provide an alternative selectivity to traditional C18 phases and are suitable for peptide mapping and the analysis of critical quality attributes (CQAs) by LC/MS.

AdvanceBio Peptide Plus columns use 2.7 µm superficially porous particles with a 100 Å pore size. The particles feature a chemically modified, hybrid endcapped C18 stationary phase with a charged surface. This surface chemistry provides improved peptide peak shape and is particularly suited to separations using formic acid as a mobile phase additive.

The 2.7 µm superficially porous particle technology is compatible with both HPLC and UHPLC instrumentation. AdvanceBio Peptide Plus columns can achieve 91% of the peak capacity of sub-2 µm particles while operating at less than half of the backpressure. The particle morphology also maintains relative peak efficiency, measured by full width at half maximum (FWHM), compared with traditional sub-2 µm particles.

Key Features of AdvanceBio Peptide Plus Columns

• Reversed-phase Hybrid endcapped C18 stationary phase with a charged surface for peptide mapping and characterization
  
• 2.7 µm superficially porous particles with 100 Å pore size
  
• Optimized for target peptides, impurities and post-translational modifications
  
• Alternative selectivity compared with traditional C18 stationary phases
  
• Designed for high-resolution peptide separations with formic acid as a mobile phase additive
  
• Maintains separation performance at higher mass loads
  
• Available in multiple column geometries
  
• Batch tested with peptide standards for quality control
  

Use the product filters below to select the appropriate Agilent AdvanceBio Peptide Plus column according to column geometry and peptide analysis requirements.