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Agilent AdvanceBio HPLC Columns for Biopharmaceutical Analysis
Agilent AdvanceBio HPLC columns are designed for the characterization and quality control of biotherapeutics. The column portfolio covers different chromatographic separation modes for monitoring critical quality attributes (CQAs) of complex biomolecules, including proteins, monoclonal antibodies, peptides and oligonucleotides.
AdvanceBio Column Types and Applications
- AdvanceBio SEC: Size exclusion chromatography for aggregate and fragment analysis of proteins and monoclonal antibodies (mAbs). Hydrophilic surface chemistry minimizes nonspecific interactions and supports good peak shape and sample recovery.
- AdvanceBio RP-mAb and Peptide Mapping: Reversed-phase chromatography for intact proteins, protein subunits and peptide mapping. These columns support analysis of amino acid sequences and post-translational modifications (PTMs). AdvanceBio Peptide Plus uses a modified charged surface for peptide separations with MS-compatible mobile phases such as formic acid.
- AdvanceBio Glycan: Amide-HILIC columns for rapid and reproducible separation and identification of released N-glycans. The columns can be combined with appropriate glycan standards and assignment tools.
- AdvanceBio Oligonucleotide: Superficially porous particles with chemically modified surfaces provide high pH and temperature stability for ion-pair reversed-phase analysis and purification of synthetic oligonucleotides, including siRNA and aptamers.
- Bio IEX, Bio MAb and AdvanceBio HIC: Ion-exchange chromatography (IEX) and hydrophobic interaction chromatography (HIC) provide separation modes for charge variant analysis and determination of drug-to-antibody ratios (DAR) in antibody drug conjugates (ADCs) under native conditions.
- AdvanceBio Surfactant Profiling: Columns for characterization of non-ionic surfactants and their degradation products.
Use the product filters below to select the appropriate Agilent AdvanceBio HPLC column according to chromatographic separation mode, biomolecule type and analytical application.
Filter products
| Product Number | Description | Price | Specifications |
|---|
| AT-5065-9946 | AdvanceBio Peptide Mapping C18 120Å 2.7µm, 0.300 x 5mm Cartridge, pk.5 |
EUR 557.00
Will be procured as soon as possible |
- Product Number: AT-5065-9946
- Manufacturer: Agilent
- Brand: AdvanceBio Peptide Mapping
- Selectivity: C18
- Selectivity Manufacturer: EC-C18
- Particle Size: 2.7 µm
- Pore Size: 120 Å
- Endcapping: yes
- Column Length: 5 mm
- Column ID: 0.300 mm
- Hardware Material: Stainless Steel
- Hardware Type: Guard Cartridge
- Application: Peptides & Proteins
- USP Code: L1
- Temperature Range: 60 °C
- pH Range: 2-8
- Particle: Silica
- Particle Shape: Spherical
- Particle Type: Core-Shell
- Surface Area: 130 m2/g
- Bonding Type: monomeric
- Accessories: Guard Cartridge
- Package Size: 5
Will be procured as soon as possible
| AT-651750-902 | AdvanceBio Peptide Mapping C18 120Å 2.7µm, 2.1 x 250mm, ea. |
EUR 1,384.00
Will be procured as soon as possible |
- Product Number: AT-651750-902
- Manufacturer: Agilent
- Brand: AdvanceBio Peptide Mapping
- Selectivity: C18
- Selectivity Manufacturer: EC-C18
- Particle Size: 2.7 µm
- Pore Size: 120 Å
- Endcapping: yes
- Column Length: 250 mm
- Column ID: 2.1 mm
- Hardware Material: Stainless Steel
- Hardware Type: Analytical Column
- Application: Peptides & Proteins
- USP Code: L1
- Temperature Range: 60 °C
- pH Range: 2-8
- Particle: Silica
- Particle Shape: Spherical
- Particle Type: Core-Shell
- Surface Area: 130 m2/g
- Bonding Type: monomeric
- Package Size: 1
Will be procured as soon as possible
| AT-651750-913 | AdvanceBio Glycan Mapping 120Å 2.7µm, 2.1 x 250mm, ea. |
EUR 1,481.00
Will be procured as soon as possible |
- Product Number: AT-651750-913
- Manufacturer: Agilent
- Brand: AdvanceBio Glycan Mapping
- Selectivity: HILIC
- Particle Size: 2.7 µm
- Pore Size: 120 Å
- Column Length: 250 mm
- Column ID: 2.1 mm
- Hardware Material: Stainless Steel
- Hardware Type: Analytical Column
- Application: Glycan Mapping
- Temperature Range: 40 °C
- pH Range: 2-7
- Particle: Silica
- Particle Shape: Spherical
- Particle Type: Core-Shell
- Surface Area: 130 m2/g
- Package Size: 1
Will be procured as soon as possible
| AT-653750-702 | AdvanceBio Oligonucleotide 120Å 2.7µm, 2.1 x 150mm, ea. |
EUR 1,394.00
Will be procured as soon as possible |
- Product Number: AT-653750-702
- Manufacturer: Agilent
- Brand: AdvanceBio Oligonucleotide
- Selectivity: C18
- Selectivity Manufacturer: Oligonucleotide
- Particle Size: 2.7 µm
- Pore Size: 120 Å
- Endcapping: yes
- Column Length: 150 mm
- Column ID: 2.1 mm
- Hardware Material: Stainless Steel
- Hardware Type: Analytical Column
- Application: Oligonucleotide
- USP Code: L1
- Temperature Range: 65 °C
- pH Range: 3-11
- Particle: Silica
- Particle Shape: Spherical
- Particle Type: Core-Shell
- Surface Area: 130 m2/g
- Bonding Type: monomeric
- Package Size: 1
Will be procured as soon as possible
| AT-653750-902 | AdvanceBio Peptide Mapping C18 120Å 2.7µm, 2.1 x 150mm, ea. |
EUR 1,193.00
Will be procured as soon as possible |
- Product Number: AT-653750-902
- Manufacturer: Agilent
- Brand: AdvanceBio Peptide Mapping
- Selectivity: C18
- Selectivity Manufacturer: EC-C18
- Particle Size: 2.7 µm
- Pore Size: 120 Å
- Endcapping: yes
- Column Length: 150 mm
- Column ID: 2.1 mm
- Hardware Material: Stainless Steel
- Hardware Type: Analytical Column
- Application: Peptides & Proteins
- USP Code: L1
- Temperature Range: 60 °C
- pH Range: 2-8
- Particle: Silica
- Particle Shape: Spherical
- Particle Type: Core-Shell
- Surface Area: 130 m2/g
- Bonding Type: monomeric
- Package Size: 1
Will be procured as soon as possible
| AT-653950-302 | AdvanceBio Peptide Mapping C18 120Å 2.7µm, 3.0 x 150mm, ea. |
EUR 1,142.00
Will be procured as soon as possible |
- Product Number: AT-653950-302
- Manufacturer: Agilent
- Brand: AdvanceBio Peptide Mapping
- Selectivity: C18
- Selectivity Manufacturer: EC-C18
- Particle Size: 2.7 µm
- Pore Size: 120 Å
- Endcapping: yes
- Column Length: 150 mm
- Column ID: 3.0 mm
- Hardware Material: Stainless Steel
- Hardware Type: Analytical Column
- Application: Peptides & Proteins
- USP Code: L1
- Temperature Range: 60 °C
- pH Range: 2-8
- Particle: Silica
- Particle Shape: Spherical
- Particle Type: Core-Shell
- Surface Area: 130 m2/g
- Bonding Type: monomeric
- Package Size: 1
Will be procured as soon as possible
| AT-653950-702 | AdvanceBio Oligonucleotide 120Å 2.7µm, 4.6 x 150mm, ea. |
EUR 1,394.00
Will be procured as soon as possible |
- Product Number: AT-653950-702
- Manufacturer: Agilent
- Brand: AdvanceBio Oligonucleotide
- Selectivity: C18
- Selectivity Manufacturer: Oligonucleotide
- Particle Size: 2.7 µm
- Pore Size: 120 Å
- Endcapping: yes
- Column Length: 150 mm
- Column ID: 4.6 mm
- Hardware Material: Stainless Steel
- Hardware Type: Analytical Column
- Application: Oligonucleotide
- USP Code: L1
- Temperature Range: 65 °C
- pH Range: 3-11
- Particle: Silica
- Particle Shape: Spherical
- Particle Type: Core-Shell
- Surface Area: 130 m2/g
- Bonding Type: monomeric
- Package Size: 1
Will be procured as soon as possible
| AT-653950-902 | AdvanceBio Peptide Mapping C18 120Å 2.7µm, 4.6 x 150mm, ea. |
EUR 1,193.00
Will be procured as soon as possible |
- Product Number: AT-653950-902
- Manufacturer: Agilent
- Brand: AdvanceBio Peptide Mapping
- Selectivity: C18
- Selectivity Manufacturer: EC-C18
- Particle Size: 2.7 µm
- Pore Size: 120 Å
- Endcapping: yes
- Column Length: 150 mm
- Column ID: 4.6 mm
- Hardware Material: Stainless Steel
- Hardware Type: Analytical Column
- Application: Peptides & Proteins
- USP Code: L1
- Temperature Range: 60 °C
- pH Range: 2-8
- Particle: Silica
- Particle Shape: Spherical
- Particle Type: Core-Shell
- Surface Area: 130 m2/g
- Bonding Type: monomeric
- Package Size: 1
Will be procured as soon as possible
| AT-655750-702 | AdvanceBio Oligonucleotide 120Å 2.7µm, 2.1 x 100mm, ea. |
EUR 1,225.00
Will be procured as soon as possible |
- Product Number: AT-655750-702
- Manufacturer: Agilent
- Brand: AdvanceBio Oligonucleotide
- Selectivity: C18
- Selectivity Manufacturer: Oligonucleotide
- Particle Size: 2.7 µm
- Pore Size: 120 Å
- Endcapping: yes
- Column Length: 100 mm
- Column ID: 2.1 mm
- Hardware Material: Stainless Steel
- Hardware Type: Analytical Column
- Application: Oligonucleotide
- USP Code: L1
- Temperature Range: 65 °C
- pH Range: 3-11
- Particle: Silica
- Particle Shape: Spherical
- Particle Type: Core-Shell
- Surface Area: 130 m2/g
- Bonding Type: monomeric
- Package Size: 1
Will be procured as soon as possible
| AT-655750-902 | AdvanceBio Peptide Mapping C18 120Å 2.7µm, 2.1 x 100mm, ea. |
EUR 1,095.00
Will be procured as soon as possible |
- Product Number: AT-655750-902
- Manufacturer: Agilent
- Brand: AdvanceBio Peptide Mapping
- Selectivity: C18
- Selectivity Manufacturer: EC-C18
- Particle Size: 2.7 µm
- Pore Size: 120 Å
- Endcapping: yes
- Column Length: 100 mm
- Column ID: 2.1 mm
- Hardware Material: Stainless Steel
- Hardware Type: Analytical Column
- Application: Peptides & Proteins
- USP Code: L1
- Temperature Range: 60 °C
- pH Range: 2-8
- Particle: Silica
- Particle Shape: Spherical
- Particle Type: Core-Shell
- Surface Area: 130 m2/g
- Bonding Type: monomeric
- Package Size: 1
Will be procured as soon as possible
| AT-655950-302 | AdvanceBio Peptide Mapping C18 120Å 2.7µm, 3.0 x 100mm, ea. |
EUR 1,093.00
Will be procured as soon as possible |
- Product Number: AT-655950-302
- Manufacturer: Agilent
- Brand: AdvanceBio Peptide Mapping
- Selectivity: C18
- Selectivity Manufacturer: EC-C18
- Particle Size: 2.7 µm
- Pore Size: 120 Å
- Endcapping: yes
- Column Length: 100 mm
- Column ID: 3.0 mm
- Hardware Material: Stainless Steel
- Hardware Type: Analytical Column
- Application: Peptides & Proteins
- USP Code: L1
- Temperature Range: 60 °C
- pH Range: 2-8
- Particle: Silica
- Particle Shape: Spherical
- Particle Type: Core-Shell
- Surface Area: 130 m2/g
- Bonding Type: monomeric
- Package Size: 1
Will be procured as soon as possible
| AT-655950-702 | AdvanceBio Oligonucleotide 120Å 2.7µm, 4.6 x 100mm, ea. |
EUR 1,225.00
Will be procured as soon as possible |
- Product Number: AT-655950-702
- Manufacturer: Agilent
- Brand: AdvanceBio Oligonucleotide
- Selectivity: C18
- Selectivity Manufacturer: Oligonucleotide
- Particle Size: 2.7 µm
- Pore Size: 120 Å
- Endcapping: yes
- Column Length: 100 mm
- Column ID: 4.6 mm
- Hardware Material: Stainless Steel
- Hardware Type: Analytical Column
- Application: Oligonucleotide
- USP Code: L1
- Temperature Range: 65 °C
- pH Range: 3-11
- Particle: Silica
- Particle Shape: Spherical
- Particle Type: Core-Shell
- Surface Area: 130 m2/g
- Bonding Type: monomeric
- Package Size: 1
Will be procured as soon as possible
| AT-655950-802 | AdvanceBio Amino Acid Analysis (AAA) 100Å 2.7µm, 4.6 x 100mm, ea. |
EUR 1,134.00
Will be procured as soon as possible |
- Product Number: AT-655950-802
- Manufacturer: Agilent
- Brand: AdvanceBio Amino Acid Analysis
- Selectivity: C18
- Selectivity Manufacturer: AAA
- Particle Size: 2.7 µm
- Pore Size: 100 Å
- Endcapping: yes
- Column Length: 100 mm
- Column ID: 4.6 mm
- Hardware Material: Stainless Steel
- Hardware Type: Analytical Column
- Application: Amino Acids
- USP Code: L1
- Temperature Range: 65 °C
- pH Range: 3-11
- Particle: Silica
- Particle Shape: Spherical
- Particle Type: Core-Shell
- Package Size: 1
Will be procured as soon as possible
| AT-659750-702 | AdvanceBio Oligonucleotide 120Å 2.7µm, 2.1 x 50mm, ea. |
EUR 997.00
Will be procured as soon as possible |
- Product Number: AT-659750-702
- Manufacturer: Agilent
- Brand: AdvanceBio Oligonucleotide
- Selectivity: C18
- Selectivity Manufacturer: Oligonucleotide
- Particle Size: 2.7 µm
- Pore Size: 120 Å
- Endcapping: yes
- Column Length: 50 mm
- Column ID: 2.1 mm
- Hardware Material: Stainless Steel
- Hardware Type: Analytical Column
- Application: Oligonucleotide
- USP Code: L1
- Temperature Range: 65 °C
- pH Range: 3-11
- Particle: Silica
- Particle Shape: Spherical
- Particle Type: Core-Shell
- Surface Area: 130 m2/g
- Bonding Type: monomeric
- Package Size: 1
Will be procured as soon as possible
| AT-659950-702 | AdvanceBio Oligonucleotide 120Å 2.7µm, 4.6 x 50mm, ea. |
EUR 997.00
Will be procured as soon as possible |
- Product Number: AT-659950-702
- Manufacturer: Agilent
- Brand: AdvanceBio Oligonucleotide
- Selectivity: C18
- Selectivity Manufacturer: Oligonucleotide
- Particle Size: 2.7 µm
- Pore Size: 120 Å
- Endcapping: yes
- Column Length: 50 mm
- Column ID: 4.6 mm
- Hardware Material: Stainless Steel
- Hardware Type: Analytical Column
- Application: Oligonucleotide
- USP Code: L1
- Temperature Range: 65 °C
- pH Range: 3-11
- Particle: Silica
- Particle Shape: Spherical
- Particle Type: Core-Shell
- Surface Area: 130 m2/g
- Bonding Type: monomeric
- Package Size: 1
Will be procured as soon as possible
| AT-671050-702 | AdvanceBio Oligonucleotide 120Å 4µm, 21.2 x 50mm, ea. |
EUR 2,813.00
Will be procured as soon as possible |
- Product Number: AT-671050-702
- Manufacturer: Agilent
- Brand: AdvanceBio Oligonucleotide
- Selectivity: C18
- Selectivity Manufacturer: Oligonucleotide
- Particle Size: 4.0 µm
- Pore Size: 120 Å
- Endcapping: yes
- Column Length: 50 mm
- Column ID: 21.2 mm
- Hardware Material: Stainless Steel
- Hardware Type: Preparative Column
- Application: Oligonucleotide
- USP Code: L1
- Temperature Range: 65 °C
- pH Range: 3-11
- Particle: Silica
- Particle Shape: Spherical
- Particle Type: Core-Shell
- Surface Area: 130 m2/g
- Bonding Type: monomeric
- Package Size: 1
Will be procured as soon as possible
| AT-671150-702 | AdvanceBio Oligonucleotide 120Å 4µm, 21.2 x 150mm, ea. | Price on request |
- Product Number: AT-671150-702
- Manufacturer: Agilent
- Brand: AdvanceBio Oligonucleotide
- Selectivity: C18
- Selectivity Manufacturer: Oligonucleotide
- Particle Size: 4.0 µm
- Pore Size: 120 Å
- Endcapping: yes
- Column Length: 150 mm
- Column ID: 21.2 mm
- Hardware Material: Stainless Steel
- Hardware Type: Preparative Column
- Application: Oligonucleotide
- USP Code: L1
- Temperature Range: 65 °C
- pH Range: 3-11
- Particle: Silica
- Particle Shape: Spherical
- Particle Type: Core-Shell
- Surface Area: 130 m2/g
- Bonding Type: monomeric
- Package Size: 1
| AT-673775-901 | AdvanceBio MS Spent Media 100Å 2.7µm, 2.1 x 150mm (PEEK-lined SS), ea. |
EUR 1,755.00
Will be procured as soon as possible |
- Product Number: AT-673775-901
- Manufacturer: Agilent
- Brand: AdvanceBio MS Spent Media
- Selectivity: HILIC
- Particle Size: 2.7 µm
- Pore Size: 100 Å
- Endcapping: no
- Column Length: 150 mm
- Column ID: 2.1 mm
- Hardware Material: PEEK-lined SS
- Hardware Type: Analytical Column
- Application: HILIC
- USP Code: L114
- Temperature Range: 80 °C
- pH Range: 3-11
- Particle: Silica
- Particle Shape: Spherical
- Particle Type: Core-Shell
- Surface Area: 95 m2/g
- Package Size: 1
Will be procured as soon as possible
| AT-675775-901 | AdvanceBio MS Spent Media 100Å 2.7µm, 2.1 x 100mm (PEEK-lined SS), ea. |
EUR 1,671.00
Will be procured as soon as possible |
- Product Number: AT-675775-901
- Manufacturer: Agilent
- Brand: AdvanceBio MS Spent Media
- Selectivity: HILIC
- Particle Size: 2.7 µm
- Pore Size: 100 Å
- Endcapping: no
- Column Length: 100 mm
- Column ID: 2.1 mm
- Hardware Material: PEEK-lined SS
- Hardware Type: Analytical Column
- Application: HILIC
- USP Code: L114
- Temperature Range: 80 °C
- pH Range: 3-11
- Particle: Silica
- Particle Shape: Spherical
- Particle Type: Core-Shell
- Surface Area: 95 m2/g
- Package Size: 1
Will be procured as soon as possible
| AT-679775-901 | AdvanceBio MS Spent Media 100Å 2.7µm, 2.1 x 50mm (PEEK-lined SS), ea. |
EUR 1,603.00
Will be procured as soon as possible |
- Product Number: AT-679775-901
- Manufacturer: Agilent
- Brand: AdvanceBio MS Spent Media
- Selectivity: HILIC
- Particle Size: 2.7 µm
- Pore Size: 100 Å
- Endcapping: no
- Column Length: 50 mm
- Column ID: 2.1 mm
- Hardware Material: PEEK-lined SS
- Hardware Type: Analytical Column
- Application: HILIC
- USP Code: L114
- Temperature Range: 80 °C
- pH Range: 3-11
- Particle: Silica
- Particle Shape: Spherical
- Particle Type: Core-Shell
- Surface Area: 95 m2/g
- Package Size: 1
Will be procured as soon as possible
| AT-680975-913 | AdvanceBio Glycan Mapping 120Å 2.7µm, 4.6 x 250mm, ea. |
EUR 1,550.00
Will be procured as soon as possible |
- Product Number: AT-680975-913
- Manufacturer: Agilent
- Brand: AdvanceBio Glycan Mapping
- Selectivity: HILIC
- Particle Size: 2.7 µm
- Pore Size: 120 Å
- Column Length: 250 mm
- Column ID: 4.6 mm
- Hardware Material: Stainless Steel
- Hardware Type: Analytical Column
- Application: Glycan Mapping
- Temperature Range: 40 °C
- pH Range: 2-7
- Particle: Silica
- Particle Shape: Spherical
- Particle Type: Core-Shell
- Surface Area: 130 m2/g
- Package Size: 1
Will be procured as soon as possible
| AT-681975-908 | AdvanceBio HIC 450Å 3.5µm, 4.6 x 30mm, ea. |
EUR 1,614.00
Will be procured as soon as possible |
- Product Number: AT-681975-908
- Manufacturer: Agilent
- Brand: AdvanceBio HIC
- Selectivity: HIC
- Particle Size: 3.5 µm
- Pore Size: 450 Å
- Column Length: 30 mm
- Column ID: 4.6 mm
- Hardware Material: Stainless Steel
- Hardware Type: Analytical Column
- Application: Hydrophobic Interaction (HIC)
- Temperature Range: 60 °C
- pH Range: 2-8
- Particle: Silica
- Particle Shape: Spherical
- Particle Type: Fully Porous
- Package Size: 1
Will be procured as soon as possible
| AT-681975-908K | AdvanceBio HIC 450Å 3.5µm, 4.6 x 30mm (MVK), ea. |
EUR 4,948.00
Will be procured as soon as possible |
- Product Number: AT-681975-908K
- Manufacturer: Agilent
- Brand: AdvanceBio HIC
- Selectivity: HIC
- Particle Size: 3.5 µm
- Pore Size: 450 Å
- Column Length: 30 mm
- Column ID: 4.6 mm
- Hardware Material: Stainless Steel
- Hardware Type: Method Validation Kit
- Application: Hydrophobic Interaction (HIC)
- Temperature Range: 60 °C
- pH Range: 2-8
- Particle: Silica
- Particle Shape: Spherical
- Particle Type: Fully Porous
- Package Size: 1
Will be procured as soon as possible
| AT-683775-913 | AdvanceBio Glycan Mapping 120Å 2.7µm, 2.1 x 150mm, ea. |
EUR 1,256.00
Will be procured as soon as possible |
- Product Number: AT-683775-913
- Manufacturer: Agilent
- Brand: AdvanceBio Glycan Mapping
- Selectivity: HILIC
- Particle Size: 2.7 µm
- Pore Size: 120 Å
- Column Length: 150 mm
- Column ID: 2.1 mm
- Hardware Material: Stainless Steel
- Hardware Type: Analytical Column
- Application: Glycan Mapping
- Temperature Range: 40 °C
- pH Range: 2-7
- Particle: Silica
- Particle Shape: Spherical
- Particle Type: Core-Shell
- Surface Area: 130 m2/g
- Package Size: 1
Will be procured as soon as possible
| AT-683975-913 | AdvanceBio Glycan Mapping 120Å 2.7µm, 4.6 x 150mm, ea. |
EUR 1,256.00
Will be procured as soon as possible |
- Product Number: AT-683975-913
- Manufacturer: Agilent
- Brand: AdvanceBio Glycan Mapping
- Selectivity: HILIC
- Particle Size: 2.7 µm
- Pore Size: 120 Å
- Column Length: 150 mm
- Column ID: 4.6 mm
- Hardware Material: Stainless Steel
- Hardware Type: Analytical Column
- Application: Glycan Mapping
- Temperature Range: 40 °C
- pH Range: 2-7
- Particle: Silica
- Particle Shape: Spherical
- Particle Type: Core-Shell
- Surface Area: 130 m2/g
- Package Size: 1
Will be procured as soon as possible