Agilent AdvanceBio HIC Bio Columns

Agilent AdvanceBio HIC (Hydrophobic Interaction Chromatography) bio columns are designed for robust and reproducible high-resolution separation of native proteins at the intact level. The columns combine ZORBAX fully porous 3.5 µm, 450 Å particles with proprietary bonding technology to provide hydrophobic selectivity for the analysis of challenging biomolecules, including monoclonal antibodies (mAbs), antibody drug conjugates (ADCs) and other recombinant proteins.

Hydrophobic interaction chromatography separates proteins according to differences in their hydrophobicity. In contrast to traditional reversed-phase chromatography, HIC uses non-denaturing mobile phase conditions, helping to preserve the biological activity of intact proteins during separation. This makes HIC suitable for analytical characterization of mAbs and ADCs as well as for downstream protein purification.

AdvanceBio HIC columns provide optimized selectivity for applications such as mAb oxidation analysis and determination of ADC drug-to-antibody ratio (DAR). The single HIC chemistry can be applied to different critical quality attributes (CQAs), reducing the requirement to screen multiple column chemistries. Shorter biocolumn formats can also decrease analysis time while maintaining separation performance.

Key Features of AdvanceBio HIC Bio Columns

• ZORBAX 3.5 µm 450 Å fully porous particle technology
  
• Designed for high-resolution separation of native intact proteins
  
• Optimized selectivity for mAb oxidation and ADC DAR analysis
  
• Suitable for monoclonal antibodies, antibody drug conjugates and recombinant proteins
  
• Non-denaturing HIC conditions help preserve biological activity of intact proteins
  
• Single chemistry for analysis of different critical quality attributes (CQAs)
  
• Each batch of media is tested with NIST mAb
  
• Individual column testing verifies packing efficiency
  
• Shorter biocolumn formats reduce analysis time while maintaining separation performance
  

Use the product filters below to select the appropriate Agilent AdvanceBio HIC bio column according to column dimensions and protein analysis requirements.