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Viva Biphenyl 300Å Series
Restek Viva LC Columns Biphenyl Header Picture

Restek Viva™ Wide Pore 300 Å Biphenyl HPLC Columns

Advanced Selectivity for Protein and Peptide Separations

The Restek Viva™ Wide Pore 300 Å Biphenyl HPLC column series is designed for high-performance reversed-phase separation of proteins, peptides, and other large biomolecules. Combining a carefully engineered 300 Å wide pore silica with Restek’s unique Biphenyl stationary phase, these columns deliver exceptional selectivity, high efficiency, and excellent reproducibility in both routine and advanced bioanalytical workflows.

The Biphenyl chemistry provides a powerful alternative selectivity compared to conventional alkyl phases, making it especially valuable for complex biomolecule mixtures where standard C18 or C8 columns do not provide sufficient resolution.

Technical Specifications

Property Specification
Stationary Phase Biphenyl (USP L11)
Pore Size 300 Å
Particle Sizes 5 µm
Surface Area ~100 m²/g
Carbon Load ~7%
End-Capped Yes
pH Range 2.5–8.0

Maximum Operating Temperature 80 °C

Find more information in the Restek Viva LC Columns Brochure.

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36 Products
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Product Number Description Price Specifications
RT-951650210 Viva Biphenyl 300Å 5µm, 4.0 x 10mm Guard Cartridge (Trident), pk.3
EUR 332.00

Will be procured as soon as possible

Restek Viva Biphenyl Guard Cartridges
  • Product Number: RT-951650210
  • Manufacturer: Restek
  • Brand: Viva
  • Selectivity: Phenyl
  • Selectivity Manufacturer: Biphenyl
  • Particle Size: 5.0 µm
  • Pore Volume: 0.82 mL/g
  • Pore Size: 300 Å
  • Carbon Load: 7.0 %
  • Endcapping: yes
  • Column Length: 10 mm
  • Column ID: 4.0 mm
  • Hardware Material: Stainless Steel
  • Hardware Type: Guard Cartridge
  • Application: Peptides & Proteins
  • USP Code: L11
  • Temperature Range: 80 °C
  • pH Range: 2.5-8
  • Particle: Silica
  • Particle Shape: Spherical
  • Particle Type: Fully Porous
  • Surface Area: 100 m2/g
  • Bonding Type: monomeric
  • Accessories: Guard Cartridge
  • Package Size: 3

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Price:
EUR 332.00

Will be procured as soon as possible

RT-951650212 Viva Biphenyl 300Å 5µm, 2.1 x 10mm Guard Cartridge (Trident), pk.3
EUR 276.00

Will be procured as soon as possible

Restek Viva Biphenyl Guard Cartridges
  • Product Number: RT-951650212
  • Manufacturer: Restek
  • Brand: Viva
  • Selectivity: Phenyl
  • Selectivity Manufacturer: Biphenyl
  • Particle Size: 5.0 µm
  • Pore Volume: 0.82 mL/g
  • Pore Size: 300 Å
  • Carbon Load: 7.0 %
  • Endcapping: yes
  • Column Length: 10 mm
  • Column ID: 2.1 mm
  • Hardware Material: Stainless Steel
  • Hardware Type: Guard Cartridge
  • Application: Peptides & Proteins
  • USP Code: L11
  • Temperature Range: 80 °C
  • pH Range: 2.5-8
  • Particle: Silica
  • Particle Shape: Spherical
  • Particle Type: Fully Porous
  • Surface Area: 100 m2/g
  • Bonding Type: monomeric
  • Accessories: Guard Cartridge
  • Package Size: 3

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Price:
EUR 276.00

Will be procured as soon as possible

RT-9516511 Viva Biphenyl 300Å 5µm, 1.0 x 100mm, ea.
EUR 706.00

Will be procured as soon as possible

Restek Viva Biphenyl LC Columns
  • Product Number: RT-9516511
  • Manufacturer: Restek
  • Brand: Viva
  • Selectivity: Phenyl
  • Selectivity Manufacturer: Biphenyl
  • Particle Size: 5.0 µm
  • Pore Volume: 0.82 mL/g
  • Pore Size: 300 Å
  • Carbon Load: 7.0 %
  • Endcapping: yes
  • Column Length: 100 mm
  • Column ID: 1.0 mm
  • Hardware Material: Stainless Steel
  • Hardware Type: Analytical Column
  • Application: Peptides & Proteins
  • USP Code: L11
  • Temperature Range: 80 °C
  • pH Range: 2.5-8
  • Particle: Silica
  • Particle Shape: Spherical
  • Particle Type: Fully Porous
  • Surface Area: 100 m2/g
  • Bonding Type: monomeric
  • Package Size: 1

Questions about the article Questions about the article To the product details To the product details

Price:
EUR 706.00

Will be procured as soon as possible

RT-9516512 Viva Biphenyl 300Å 5µm, 2.1 x 100mm, ea.
EUR 685.00

Will be procured as soon as possible

Restek Viva Biphenyl LC Columns
  • Product Number: RT-9516512
  • Manufacturer: Restek
  • Brand: Viva
  • Selectivity: Phenyl
  • Selectivity Manufacturer: Biphenyl
  • Particle Size: 5.0 µm
  • Pore Volume: 0.82 mL/g
  • Pore Size: 300 Å
  • Carbon Load: 7.0 %
  • Endcapping: yes
  • Column Length: 100 mm
  • Column ID: 2.1 mm
  • Hardware Material: Stainless Steel
  • Hardware Type: Analytical Column
  • Application: Peptides & Proteins
  • USP Code: L11
  • Temperature Range: 80 °C
  • pH Range: 2.5-8
  • Particle: Silica
  • Particle Shape: Spherical
  • Particle Type: Fully Porous
  • Surface Area: 100 m2/g
  • Bonding Type: monomeric
  • Package Size: 1

Questions about the article Questions about the article To the product details To the product details

Price:
EUR 685.00

Will be procured as soon as possible

RT-9516512-700 Viva Biphenyl 300Å 5µm, 2.1 x 100mm (Trident), ea. Price on request
Restek Viva Biphenyl LC Columns
  • Product Number: RT-9516512-700
  • Manufacturer: Restek
  • Brand: Viva
  • Selectivity: Phenyl
  • Selectivity Manufacturer: Biphenyl
  • Particle Size: 5.0 µm
  • Pore Volume: 0.82 mL/g
  • Pore Size: 300 Å
  • Carbon Load: 7.0 %
  • Endcapping: yes
  • Column Length: 100 mm
  • Column ID: 2.1 mm
  • Hardware Material: Stainless Steel
  • Hardware Type: Analytical Column
  • Application: Peptides & Proteins
  • USP Code: L11
  • Temperature Range: 80 °C
  • pH Range: 2.5-8
  • Particle: Silica
  • Particle Shape: Spherical
  • Particle Type: Fully Porous
  • Surface Area: 100 m2/g
  • Bonding Type: monomeric
  • Package Size: 1

Questions about the article Questions about the article To the product details To the product details

Price on request
RT-9516515 Viva Biphenyl 300Å 5µm, 4.6 x 100mm, ea.
EUR 1,132.00

Will be procured as soon as possible

Restek Viva Biphenyl LC Columns
  • Product Number: RT-9516515
  • Manufacturer: Restek
  • Brand: Viva
  • Selectivity: Phenyl
  • Selectivity Manufacturer: Biphenyl
  • Particle Size: 5.0 µm
  • Pore Volume: 0.82 mL/g
  • Pore Size: 300 Å
  • Carbon Load: 7.0 %
  • Endcapping: yes
  • Column Length: 100 mm
  • Column ID: 4.6 mm
  • Hardware Material: Stainless Steel
  • Hardware Type: Analytical Column
  • Application: Peptides & Proteins
  • USP Code: L11
  • Temperature Range: 80 °C
  • pH Range: 2.5-8
  • Particle: Silica
  • Particle Shape: Spherical
  • Particle Type: Fully Porous
  • Surface Area: 100 m2/g
  • Bonding Type: monomeric
  • Package Size: 1

Questions about the article Questions about the article To the product details To the product details

Price:
EUR 1,132.00

Will be procured as soon as possible

RT-9516515-700 Viva Biphenyl 300Å 5µm, 4.6 x 100mm (Trident), ea. Price on request
Restek Viva Biphenyl LC Columns
  • Product Number: RT-9516515-700
  • Manufacturer: Restek
  • Brand: Viva
  • Selectivity: Phenyl
  • Selectivity Manufacturer: Biphenyl
  • Particle Size: 5.0 µm
  • Pore Volume: 0.82 mL/g
  • Pore Size: 300 Å
  • Carbon Load: 7.0 %
  • Endcapping: yes
  • Column Length: 100 mm
  • Column ID: 4.6 mm
  • Hardware Material: Stainless Steel
  • Hardware Type: Analytical Column
  • Application: Peptides & Proteins
  • USP Code: L11
  • Temperature Range: 80 °C
  • pH Range: 2.5-8
  • Particle: Silica
  • Particle Shape: Spherical
  • Particle Type: Fully Porous
  • Surface Area: 100 m2/g
  • Bonding Type: monomeric
  • Package Size: 1

Questions about the article Questions about the article To the product details To the product details

Price on request
RT-951651E Viva Biphenyl 300Å 5µm, 3.0 x 100mm, ea.
EUR 1,003.00

Will be procured as soon as possible

Restek Viva Biphenyl LC Columns
  • Product Number: RT-951651E
  • Manufacturer: Restek
  • Brand: Viva
  • Selectivity: Phenyl
  • Selectivity Manufacturer: Biphenyl
  • Particle Size: 5.0 µm
  • Pore Volume: 0.82 mL/g
  • Pore Size: 300 Å
  • Carbon Load: 7.0 %
  • Endcapping: yes
  • Column Length: 100 mm
  • Column ID: 3.0 mm
  • Hardware Material: Stainless Steel
  • Hardware Type: Analytical Column
  • Application: Peptides & Proteins
  • USP Code: L11
  • Temperature Range: 80 °C
  • pH Range: 2.5-8
  • Particle: Silica
  • Particle Shape: Spherical
  • Particle Type: Fully Porous
  • Surface Area: 100 m2/g
  • Bonding Type: monomeric
  • Package Size: 1

Questions about the article Questions about the article To the product details To the product details

Price:
EUR 1,003.00

Will be procured as soon as possible

RT-9516522 Viva Biphenyl 300Å 5µm, 2.1 x 200mm, ea.
EUR 1,115.00

Will be procured as soon as possible

Restek Viva Biphenyl LC Columns
  • Product Number: RT-9516522
  • Manufacturer: Restek
  • Brand: Viva
  • Selectivity: Phenyl
  • Selectivity Manufacturer: Biphenyl
  • Particle Size: 5.0 µm
  • Pore Volume: 0.82 mL/g
  • Pore Size: 300 Å
  • Carbon Load: 7.0 %
  • Endcapping: yes
  • Column Length: 200 mm
  • Column ID: 2.1 mm
  • Hardware Material: Stainless Steel
  • Hardware Type: Analytical Column
  • Application: Peptides & Proteins
  • USP Code: L11
  • Temperature Range: 80 °C
  • pH Range: 2.5-8
  • Particle: Silica
  • Particle Shape: Spherical
  • Particle Type: Fully Porous
  • Surface Area: 100 m2/g
  • Bonding Type: monomeric
  • Package Size: 1

Questions about the article Questions about the article To the product details To the product details

Price:
EUR 1,115.00

Will be procured as soon as possible

RT-9516522-700 Viva Biphenyl 300Å 5µm, 2.1 x 200mm (Trident), ea. Price on request
Restek Viva Biphenyl LC Columns
  • Product Number: RT-9516522-700
  • Manufacturer: Restek
  • Brand: Viva
  • Selectivity: Phenyl
  • Selectivity Manufacturer: Biphenyl
  • Particle Size: 5.0 µm
  • Pore Volume: 0.82 mL/g
  • Pore Size: 300 Å
  • Carbon Load: 7.0 %
  • Endcapping: yes
  • Column Length: 200 mm
  • Column ID: 2.1 mm
  • Hardware Material: Stainless Steel
  • Hardware Type: Analytical Column
  • Application: Peptides & Proteins
  • USP Code: L11
  • Temperature Range: 80 °C
  • pH Range: 2.5-8
  • Particle: Silica
  • Particle Shape: Spherical
  • Particle Type: Fully Porous
  • Surface Area: 100 m2/g
  • Bonding Type: monomeric
  • Package Size: 1

Questions about the article Questions about the article To the product details To the product details

Price on request
RT-9516525 Viva Biphenyl 300Å 5µm, 4.6 x 200mm, ea.
EUR 1,518.00

Will be procured as soon as possible

Restek Viva Biphenyl LC Columns
  • Product Number: RT-9516525
  • Manufacturer: Restek
  • Brand: Viva
  • Selectivity: Phenyl
  • Selectivity Manufacturer: Biphenyl
  • Particle Size: 5.0 µm
  • Pore Volume: 0.82 mL/g
  • Pore Size: 300 Å
  • Carbon Load: 7.0 %
  • Endcapping: yes
  • Column Length: 200 mm
  • Column ID: 4.6 mm
  • Hardware Material: Stainless Steel
  • Hardware Type: Analytical Column
  • Application: Peptides & Proteins
  • USP Code: L11
  • Temperature Range: 80 °C
  • pH Range: 2.5-8
  • Particle: Silica
  • Particle Shape: Spherical
  • Particle Type: Fully Porous
  • Surface Area: 100 m2/g
  • Bonding Type: monomeric
  • Package Size: 1

Questions about the article Questions about the article To the product details To the product details

Price:
EUR 1,518.00

Will be procured as soon as possible

RT-9516525-700 Viva Biphenyl 300Å 5µm, 4.6 x 200mm (Trident), ea. Price on request
Restek Viva Biphenyl LC Columns
  • Product Number: RT-9516525-700
  • Manufacturer: Restek
  • Brand: Viva
  • Selectivity: Phenyl
  • Selectivity Manufacturer: Biphenyl
  • Particle Size: 5.0 µm
  • Pore Volume: 0.82 mL/g
  • Pore Size: 300 Å
  • Carbon Load: 7.0 %
  • Endcapping: yes
  • Column Length: 200 mm
  • Column ID: 4.6 mm
  • Hardware Material: Stainless Steel
  • Hardware Type: Analytical Column
  • Application: Peptides & Proteins
  • USP Code: L11
  • Temperature Range: 80 °C
  • pH Range: 2.5-8
  • Particle: Silica
  • Particle Shape: Spherical
  • Particle Type: Fully Porous
  • Surface Area: 100 m2/g
  • Bonding Type: monomeric
  • Package Size: 1

Questions about the article Questions about the article To the product details To the product details

Price on request
RT-951652E Viva Biphenyl 300Å 5µm, 3.0 x 200mm, ea.
EUR 1,708.00

Will be procured as soon as possible

Restek Viva Biphenyl LC Columns
  • Product Number: RT-951652E
  • Manufacturer: Restek
  • Brand: Viva
  • Selectivity: Phenyl
  • Selectivity Manufacturer: Biphenyl
  • Particle Size: 5.0 µm
  • Pore Volume: 0.82 mL/g
  • Pore Size: 300 Å
  • Carbon Load: 7.0 %
  • Endcapping: yes
  • Column Length: 200 mm
  • Column ID: 3.0 mm
  • Hardware Material: Stainless Steel
  • Hardware Type: Analytical Column
  • Application: Peptides & Proteins
  • USP Code: L11
  • Temperature Range: 80 °C
  • pH Range: 2.5-8
  • Particle: Silica
  • Particle Shape: Spherical
  • Particle Type: Fully Porous
  • Surface Area: 100 m2/g
  • Bonding Type: monomeric
  • Package Size: 1

Questions about the article Questions about the article To the product details To the product details

Price:
EUR 1,708.00

Will be procured as soon as possible

RT-9516532 Viva Biphenyl 300Å 5µm, 2.1 x 30mm, ea.
EUR 617.00

Will be procured as soon as possible

Restek Viva Biphenyl LC Columns
  • Product Number: RT-9516532
  • Manufacturer: Restek
  • Brand: Viva
  • Selectivity: Phenyl
  • Selectivity Manufacturer: Biphenyl
  • Particle Size: 5.0 µm
  • Pore Volume: 0.82 mL/g
  • Pore Size: 300 Å
  • Carbon Load: 7.0 %
  • Endcapping: yes
  • Column Length: 30 mm
  • Column ID: 2.1 mm
  • Hardware Material: Stainless Steel
  • Hardware Type: Analytical Column
  • Application: Peptides & Proteins
  • USP Code: L11
  • Temperature Range: 80 °C
  • pH Range: 2.5-8
  • Particle: Silica
  • Particle Shape: Spherical
  • Particle Type: Fully Porous
  • Surface Area: 100 m2/g
  • Bonding Type: monomeric
  • Package Size: 1

Questions about the article Questions about the article To the product details To the product details

Price:
EUR 617.00

Will be procured as soon as possible

RT-9516532-700 Viva Biphenyl 300Å 5µm, 2.1 x 30mm (Trident), ea. Price on request
Restek Viva Biphenyl LC Columns
  • Product Number: RT-9516532-700
  • Manufacturer: Restek
  • Brand: Viva
  • Selectivity: Phenyl
  • Selectivity Manufacturer: Biphenyl
  • Particle Size: 5.0 µm
  • Pore Volume: 0.82 mL/g
  • Pore Size: 300 Å
  • Carbon Load: 7.0 %
  • Endcapping: yes
  • Column Length: 30 mm
  • Column ID: 2.1 mm
  • Hardware Material: Stainless Steel
  • Hardware Type: Analytical Column
  • Application: Peptides & Proteins
  • USP Code: L11
  • Temperature Range: 80 °C
  • pH Range: 2.5-8
  • Particle: Silica
  • Particle Shape: Spherical
  • Particle Type: Fully Porous
  • Surface Area: 100 m2/g
  • Bonding Type: monomeric
  • Package Size: 1

Questions about the article Questions about the article To the product details To the product details

Price on request
RT-9516535 Viva Biphenyl 300Å 5µm, 4.6 x 30mm, ea.
EUR 712.00

Will be procured as soon as possible

Restek Viva Biphenyl LC Columns
  • Product Number: RT-9516535
  • Manufacturer: Restek
  • Brand: Viva
  • Selectivity: Phenyl
  • Selectivity Manufacturer: Biphenyl
  • Particle Size: 5.0 µm
  • Pore Volume: 0.82 mL/g
  • Pore Size: 300 Å
  • Carbon Load: 7.0 %
  • Endcapping: yes
  • Column Length: 30 mm
  • Column ID: 4.6 mm
  • Hardware Material: Stainless Steel
  • Hardware Type: Analytical Column
  • Application: Peptides & Proteins
  • USP Code: L11
  • Temperature Range: 80 °C
  • pH Range: 2.5-8
  • Particle: Silica
  • Particle Shape: Spherical
  • Particle Type: Fully Porous
  • Surface Area: 100 m2/g
  • Bonding Type: monomeric
  • Package Size: 1

Questions about the article Questions about the article To the product details To the product details

Price:
EUR 712.00

Will be procured as soon as possible

RT-9516535-700 Viva Biphenyl 300Å 5µm, 4.6 x 30mm (Trident), ea. Price on request
Restek Viva Biphenyl LC Columns
  • Product Number: RT-9516535-700
  • Manufacturer: Restek
  • Brand: Viva
  • Selectivity: Phenyl
  • Selectivity Manufacturer: Biphenyl
  • Particle Size: 5.0 µm
  • Pore Volume: 0.82 mL/g
  • Pore Size: 300 Å
  • Carbon Load: 7.0 %
  • Endcapping: yes
  • Column Length: 30 mm
  • Column ID: 4.6 mm
  • Hardware Material: Stainless Steel
  • Hardware Type: Analytical Column
  • Application: Peptides & Proteins
  • USP Code: L11
  • Temperature Range: 80 °C
  • pH Range: 2.5-8
  • Particle: Silica
  • Particle Shape: Spherical
  • Particle Type: Fully Porous
  • Surface Area: 100 m2/g
  • Bonding Type: monomeric
  • Package Size: 1

Questions about the article Questions about the article To the product details To the product details

Price on request
RT-951653E Viva Biphenyl 300Å 5µm, 3.0 x 30mm, ea.
EUR 617.00

Will be procured as soon as possible

Restek Viva Biphenyl LC Columns
  • Product Number: RT-951653E
  • Manufacturer: Restek
  • Brand: Viva
  • Selectivity: Phenyl
  • Selectivity Manufacturer: Biphenyl
  • Particle Size: 5.0 µm
  • Pore Volume: 0.82 mL/g
  • Pore Size: 300 Å
  • Carbon Load: 7.0 %
  • Endcapping: yes
  • Column Length: 30 mm
  • Column ID: 3.0 mm
  • Hardware Material: Stainless Steel
  • Hardware Type: Analytical Column
  • Application: Peptides & Proteins
  • USP Code: L11
  • Temperature Range: 80 °C
  • pH Range: 2.5-8
  • Particle: Silica
  • Particle Shape: Spherical
  • Particle Type: Fully Porous
  • Surface Area: 100 m2/g
  • Bonding Type: monomeric
  • Package Size: 1

Questions about the article Questions about the article To the product details To the product details

Price:
EUR 617.00

Will be procured as soon as possible

RT-9516551 Viva Biphenyl 300Å 5µm, 1.0 x 50mm, ea.
EUR 653.00

Will be procured as soon as possible

Restek Viva Biphenyl LC Columns
  • Product Number: RT-9516551
  • Manufacturer: Restek
  • Brand: Viva
  • Selectivity: Phenyl
  • Selectivity Manufacturer: Biphenyl
  • Particle Size: 5.0 µm
  • Pore Volume: 0.82 mL/g
  • Pore Size: 300 Å
  • Carbon Load: 7.0 %
  • Endcapping: yes
  • Column Length: 50 mm
  • Column ID: 1.0 mm
  • Hardware Material: Stainless Steel
  • Hardware Type: Analytical Column
  • Application: Peptides & Proteins
  • USP Code: L11
  • Temperature Range: 80 °C
  • pH Range: 2.5-8
  • Particle: Silica
  • Particle Shape: Spherical
  • Particle Type: Fully Porous
  • Surface Area: 100 m2/g
  • Bonding Type: monomeric
  • Package Size: 1

Questions about the article Questions about the article To the product details To the product details

Price:
EUR 653.00

Will be procured as soon as possible

RT-9516552 Viva Biphenyl 300Å 5µm, 2.1 x 50mm, ea.
EUR 617.00

Will be procured as soon as possible

Restek Viva Biphenyl LC Columns
  • Product Number: RT-9516552
  • Manufacturer: Restek
  • Brand: Viva
  • Selectivity: Phenyl
  • Selectivity Manufacturer: Biphenyl
  • Particle Size: 5.0 µm
  • Pore Volume: 0.82 mL/g
  • Pore Size: 300 Å
  • Carbon Load: 7.0 %
  • Endcapping: yes
  • Column Length: 50 mm
  • Column ID: 2.1 mm
  • Hardware Material: Stainless Steel
  • Hardware Type: Analytical Column
  • Application: Peptides & Proteins
  • USP Code: L11
  • Temperature Range: 80 °C
  • pH Range: 2.5-8
  • Particle: Silica
  • Particle Shape: Spherical
  • Particle Type: Fully Porous
  • Surface Area: 100 m2/g
  • Bonding Type: monomeric
  • Package Size: 1

Questions about the article Questions about the article To the product details To the product details

Price:
EUR 617.00

Will be procured as soon as possible

RT-9516552-700 Viva Biphenyl 300Å 5µm, 2.1 x 50mm (Trident), ea. Price on request
Restek Viva Biphenyl LC Columns
  • Product Number: RT-9516552-700
  • Manufacturer: Restek
  • Brand: Viva
  • Selectivity: Phenyl
  • Selectivity Manufacturer: Biphenyl
  • Particle Size: 5.0 µm
  • Pore Volume: 0.82 mL/g
  • Pore Size: 300 Å
  • Carbon Load: 7.0 %
  • Endcapping: yes
  • Column Length: 50 mm
  • Column ID: 2.1 mm
  • Hardware Material: Stainless Steel
  • Hardware Type: Analytical Column
  • Application: Peptides & Proteins
  • USP Code: L11
  • Temperature Range: 80 °C
  • pH Range: 2.5-8
  • Particle: Silica
  • Particle Shape: Spherical
  • Particle Type: Fully Porous
  • Surface Area: 100 m2/g
  • Bonding Type: monomeric
  • Package Size: 1

Questions about the article Questions about the article To the product details To the product details

Price on request
RT-9516555 Viva Biphenyl 300Å 5µm, 4.6 x 50mm, ea.
EUR 891.00

Will be procured as soon as possible

Restek Viva Biphenyl LC Columns
  • Product Number: RT-9516555
  • Manufacturer: Restek
  • Brand: Viva
  • Selectivity: Phenyl
  • Selectivity Manufacturer: Biphenyl
  • Particle Size: 5.0 µm
  • Pore Volume: 0.82 mL/g
  • Pore Size: 300 Å
  • Carbon Load: 7.0 %
  • Endcapping: yes
  • Column Length: 50 mm
  • Column ID: 4.6 mm
  • Hardware Material: Stainless Steel
  • Hardware Type: Analytical Column
  • Application: Peptides & Proteins
  • USP Code: L11
  • Temperature Range: 80 °C
  • pH Range: 2.5-8
  • Particle: Silica
  • Particle Shape: Spherical
  • Particle Type: Fully Porous
  • Surface Area: 100 m2/g
  • Bonding Type: monomeric
  • Package Size: 1

Questions about the article Questions about the article To the product details To the product details

Price:
EUR 891.00

Will be procured as soon as possible

RT-9516555-700 Viva Biphenyl 300Å 5µm, 4.6 x 50mm (Trident), ea. Price on request
Restek Viva Biphenyl LC Columns
  • Product Number: RT-9516555-700
  • Manufacturer: Restek
  • Brand: Viva
  • Selectivity: Phenyl
  • Selectivity Manufacturer: Biphenyl
  • Particle Size: 5.0 µm
  • Pore Volume: 0.82 mL/g
  • Pore Size: 300 Å
  • Carbon Load: 7.0 %
  • Endcapping: yes
  • Column Length: 50 mm
  • Column ID: 4.6 mm
  • Hardware Material: Stainless Steel
  • Hardware Type: Analytical Column
  • Application: Peptides & Proteins
  • USP Code: L11
  • Temperature Range: 80 °C
  • pH Range: 2.5-8
  • Particle: Silica
  • Particle Shape: Spherical
  • Particle Type: Fully Porous
  • Surface Area: 100 m2/g
  • Bonding Type: monomeric
  • Package Size: 1

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Price on request
RT-951655E Viva Biphenyl 300Å 5µm, 3.0 x 50mm, ea.
EUR 683.00

Will be procured as soon as possible

Restek Viva Biphenyl LC Columns
  • Product Number: RT-951655E
  • Manufacturer: Restek
  • Brand: Viva
  • Selectivity: Phenyl
  • Selectivity Manufacturer: Biphenyl
  • Particle Size: 5.0 µm
  • Pore Volume: 0.82 mL/g
  • Pore Size: 300 Å
  • Carbon Load: 7.0 %
  • Endcapping: yes
  • Column Length: 50 mm
  • Column ID: 3.0 mm
  • Hardware Material: Stainless Steel
  • Hardware Type: Analytical Column
  • Application: Peptides & Proteins
  • USP Code: L11
  • Temperature Range: 80 °C
  • pH Range: 2.5-8
  • Particle: Silica
  • Particle Shape: Spherical
  • Particle Type: Fully Porous
  • Surface Area: 100 m2/g
  • Bonding Type: monomeric
  • Package Size: 1

Questions about the article Questions about the article To the product details To the product details

Price:
EUR 683.00

Will be procured as soon as possible

RT-9516561 Viva Biphenyl 300Å 5µm, 1.0 x 150mm, ea.
EUR 760.00

Will be procured as soon as possible

Restek Viva Biphenyl LC Columns
  • Product Number: RT-9516561
  • Manufacturer: Restek
  • Brand: Viva
  • Selectivity: Phenyl
  • Selectivity Manufacturer: Biphenyl
  • Particle Size: 5.0 µm
  • Pore Volume: 0.82 mL/g
  • Pore Size: 300 Å
  • Carbon Load: 7.0 %
  • Endcapping: yes
  • Column Length: 150 mm
  • Column ID: 1.0 mm
  • Hardware Material: Stainless Steel
  • Hardware Type: Analytical Column
  • Application: Peptides & Proteins
  • USP Code: L11
  • Temperature Range: 80 °C
  • pH Range: 2.5-8
  • Particle: Silica
  • Particle Shape: Spherical
  • Particle Type: Fully Porous
  • Surface Area: 100 m2/g
  • Bonding Type: monomeric
  • Package Size: 1

Questions about the article Questions about the article To the product details To the product details

Price:
EUR 760.00

Will be procured as soon as possible

Optimized 300 Å Wide Pore Silica for Biomolecules

Efficient separation of proteins and peptides requires full accessibility of the stationary phase surface. The Viva™ Wide Pore silica is engineered with a narrow pore size distribution centered at 300 Å, ensuring that a high proportion of the internal surface is available for interaction with large molecules.

This optimized pore structure improves:

  • Mass transfer for high molecular weight analytes
  • Surface accessibility for proteins and peptides
  • Peak shape and chromatographic efficiency
  • Reproducibility in bioanalytical methods

Compared to conventional wide-pore materials, the Viva silica design minimizes inaccessible micropore volume, resulting in more efficient and predictable biomolecule separations.

Unique Biphenyl Selectivity for Challenging Separations

The Viva™ Biphenyl stationary phase (USP L11) offers a distinct separation mechanism based on enhanced π–π interactions, dipole interactions, and polarizability. This provides strong orthogonal selectivity compared to traditional reversed-phase phases such as C18 or C8.

For protein and peptide analysis, this results in:

  • Improved resolution of structurally similar peptides
  • Enhanced separation of aromatic and conjugated sequences
  • Stronger retention of polarizable biomolecules
  • Complementary selectivity in method development workflows

The Biphenyl phase is particularly effective when analyzing complex peptide mixtures or when critical pair separations cannot be achieved using conventional hydrophobic phases.

Ideal for Proteins, Peptides, and LC-MS Workflows

The Viva™ Wide Pore Biphenyl column is well suited for modern bioanalytical applications, including:

  • Peptide mapping and characterization
  • Protein digest analysis
  • Intact protein separations
  • Biopharmaceutical quality control
  • Monoclonal antibody research
  • Biomarker and proteomics studies
  • LC-MS method development

Its MS-friendly selectivity profile allows efficient separations using simple mobile phase systems, helping to reduce ion suppression and improve detection sensitivity.

Key Features and Benefits

  • 300 Å wide pore silica optimized for biomolecules
  • Narrow pore size distribution for maximum surface accessibility
  • Unique Biphenyl stationary phase (USP L11)
  • Strong orthogonal selectivity vs. C18/C8 phases
  • Enhanced retention of aromatic and polarizable compounds
  • Excellent peak shape and reproducibility
  • High recovery of peptides and proteins
  • Compatible with LC-UV and LC-MS detection
  • Robust performance for routine and research applications

Reliable Performance for Bioanalytical Laboratories

Restek manufactures Viva™ columns using tightly controlled production processes from silica synthesis through final column packing. This ensures consistent batch-to-batch performance, reproducible retention times, and long-term column stability—critical factors in regulated bioanalytical environments.

The combination of advanced wide pore silica and Biphenyl selectivity makes Viva™ Biphenyl columns a powerful tool for developing robust and highly selective protein and peptide methods.

The Next Level of Selectivity for Biomolecule Analysis

The Restek Viva™ Wide Pore 300 Å Biphenyl HPLC column combines advanced pore engineering with a uniquely selective stationary phase, offering a powerful alternative for protein and peptide separations. When conventional C18 or C8 phases reach their limits, Biphenyl chemistry provides the orthogonal selectivity needed to resolve complex biomolecular mixtures with confidence.

Whether for method development, routine QC, or advanced LC-MS workflows, Viva™ Biphenyl columns deliver the resolution, robustness, and reproducibility required in modern bioanalysis.

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