Agilent AdvanceBio Glycan Mapping Columns

AdvanceBio Glycan Mapping Series

Agilent AdvanceBio Glycan Mapping Columns

Agilent AdvanceBio Glycan Mapping columns are designed for the HILIC separation and identification of glycans during biomolecule characterization. Glycosylation is an important post-translational modification, and detailed glycan mapping can be used to characterize glycosylation profiles and detect changes resulting from process variables. The amide-based stationary phase provides hydrophilic interaction chromatography (HILIC) for the separation of labeled and unlabeled glycans.

AdvanceBio Glycan Mapping columns are available with 1.8 µm fully porous particles or 2.7 µm superficially porous particles. The 1.8 µm configuration is designed for fast UHPLC glycan analysis and can provide glycan maps in less than 10 minutes. The 2.7 µm superficially porous configuration combines high separation efficiency with lower backpressure and is also available in longer 250 mm columns for high-resolution glycan separations. They are compatible with mobile phases used for fluorescence and mass spectrometry (MS) detection. This enables HILIC analysis of labeled N-glycans as well as detailed evaluation and quantitation of glycan profiles.

Key Features of AdvanceBio Glycan Mapping Columns

Amide-based HILIC stationary phase with hydrophilic bonding for glycan separations
Suitable for separation and characterization of labeled and unlabeled glycans
1.8 µm fully porous particles for high-resolution and high-throughput UHPLC analysis up to 1200 bar
2.7 µm superficially porous particles for high efficiency with reduced backpressure up to 600 bar
Operating pH range of 2–7
Compatible with fluorescence and mass spectrometry detection
Each media batch is QA tested using a 2-AB labeled IgG N-linked glycan sample

Use the product filters below to select the appropriate Agilent AdvanceBio Glycan Mapping column according to particle size, column dimensions and glycan analysis requirements.

11 Produits

AdvanceBio Glycan Mapping 120Å 2.7µm, 2.1 x 250mm, ea.

Agilent AdvanceBio Glycan Mapping 120Å 2.7µm, 2.1 x 250mm, ea.

Art.-Nr.: AT-651750-913

EUR 1,481.00